Metagenomic analysis of the ferret fecal viral flora

Saskia L Smits; V Stalin Raj; Minoushka D Oduber; Claudia M E Schapendonk; Rogier Bodewes; Lisette Provacia; Koert J Stittelaar; Albert D M E Osterhaus; Bart L Haagmans

doi:10.1371/journal.pone.0071595

Metagenomic analysis of the ferret fecal viral flora

Saskia L Smits, V Stalin Raj, Minoushka D Oduber, Claudia M E Schapendonk, Rogier Bodewes, Lisette Provacia, Koert J Stittelaar, Albert D M E Osterhaus, Bart L Haagmans

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Abstract

Ferrets are widely used as a small animal model for a number of viral infections, including influenza A virus and SARS coronavirus. To further analyze the microbiological status of ferrets, their fecal viral flora was studied using a metagenomics approach. Novel viruses from the families Picorna-, Papilloma-, and Anelloviridae as well as known viruses from the families Astro-, Corona-, Parvo-, and Hepeviridae were identified in different ferret cohorts. Ferret kobu- and hepatitis E virus were mainly present in human household ferrets, whereas coronaviruses were found both in household as well as farm ferrets. Our studies illuminate the viral diversity found in ferrets and provide tools to prescreen for newly identified viruses that potentially could influence disease outcome of experimental virus infections in ferrets.

Original language	English
Pages (from-to)	e71595
Journal	PLoS One
Volume	8
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0071595
Publication status	Published - 2013

Keywords

Amino Acid Sequence
Animals
Base Sequence
Feces
Ferrets
Genetic Variation
Genome, Viral
Humans
Metagenomics
Molecular Sequence Data
Phylogeny
Sequence Homology, Amino Acid
Viruses

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title = "Metagenomic analysis of the ferret fecal viral flora",

abstract = "Ferrets are widely used as a small animal model for a number of viral infections, including influenza A virus and SARS coronavirus. To further analyze the microbiological status of ferrets, their fecal viral flora was studied using a metagenomics approach. Novel viruses from the families Picorna-, Papilloma-, and Anelloviridae as well as known viruses from the families Astro-, Corona-, Parvo-, and Hepeviridae were identified in different ferret cohorts. Ferret kobu- and hepatitis E virus were mainly present in human household ferrets, whereas coronaviruses were found both in household as well as farm ferrets. Our studies illuminate the viral diversity found in ferrets and provide tools to prescreen for newly identified viruses that potentially could influence disease outcome of experimental virus infections in ferrets.",

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AU - Raj, V Stalin

AU - Oduber, Minoushka D

AU - Schapendonk, Claudia M E

AU - Bodewes, Rogier

AU - Provacia, Lisette

AU - Stittelaar, Koert J

AU - Osterhaus, Albert D M E

AU - Haagmans, Bart L

PY - 2013

Y1 - 2013

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AB - Ferrets are widely used as a small animal model for a number of viral infections, including influenza A virus and SARS coronavirus. To further analyze the microbiological status of ferrets, their fecal viral flora was studied using a metagenomics approach. Novel viruses from the families Picorna-, Papilloma-, and Anelloviridae as well as known viruses from the families Astro-, Corona-, Parvo-, and Hepeviridae were identified in different ferret cohorts. Ferret kobu- and hepatitis E virus were mainly present in human household ferrets, whereas coronaviruses were found both in household as well as farm ferrets. Our studies illuminate the viral diversity found in ferrets and provide tools to prescreen for newly identified viruses that potentially could influence disease outcome of experimental virus infections in ferrets.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Feces

KW - Ferrets

KW - Genetic Variation

KW - Genome, Viral

KW - Humans

KW - Metagenomics

KW - Molecular Sequence Data

KW - Phylogeny

KW - Sequence Homology, Amino Acid

KW - Viruses

U2 - 10.1371/journal.pone.0071595

DO - 10.1371/journal.pone.0071595

M3 - Article

C2 - 23977082

SN - 1932-6203

VL - 8

SP - e71595

JO - PLoS One

JF - PLoS One

IS - 8

ER -

Metagenomic analysis of the ferret fecal viral flora

Abstract

Keywords

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