Melanin biosynthesis pathway in Agaricus bisporus mushrooms

A. Weijn, S. Bastiaan-Net, H.J. Wichers, J.J. Mes

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

With the full genome sequence of Agaricus bisporus available, it was possible to investigate the genes involved in the melanin biosynthesis pathway of button mushrooms. Based on different BLAST and alignments, genes were identified in the genome which are postulated to be involved in this pathway. Seven housekeeping genes were tested of which 18S rRNA was the only housekeeping gene that was stably expressed in various tissues of different developmental stages. Gene expression was determined for most gene homologs (26 genes) involved in the melanin pathway. Of the analysed genes, those encoding polyphenol oxidase (PPO), the PPO co-factor L-chain (unique for A. bisporus), and a putative transcription factor (photoregulator B) were among the highest expressed in skin tissue. An in depth look was taken at the clustering of several PPO genes and the PPO co-factor gene on chromosome 5, which showed that almost 25% of the protein encoding genes in this cluster have a conserved NACHT and WD40 domain or a P-loop nucleoside triphosphate hydrolase. This article will be the start for an in depth study of the melanin pathway and its role in quality losses of this economically important product. © 2012 Elsevier Inc.
Original languageEnglish
Pages (from-to)42-53
Number of pages12
JournalFungal Genetics and Biology
Volume55
DOIs
Publication statusPublished - 1 Jun 2013
Externally publishedYes

Keywords

  • Agaricus bisporus
  • Gene expression
  • Melanin biosynthesis pathway
  • Polyphenol oxidase
  • Tyrosinase
  • catechol oxidase
  • hydrolase
  • nucleoside triphosphate hydrolase
  • photoregulator B
  • RNA 18S
  • transcription factor
  • unclassified drug
  • Actin gene
  • article
  • chromosome 5
  • fungal genome
  • gene cluster
  • gene expression
  • genetic conservation
  • genetic identification
  • GPD gene
  • housekeeping gene
  • melanogenesis
  • nonhuman
  • nucleotide sequence
  • PgkA gene
  • PkiA gene
  • priority journal
  • quantitative analysis
  • real time polymerase chain reaction
  • RNA 18S gene
  • TefA gene
  • Tubulin gene

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