Measuring plant root traits under controlled and field conditions: Step-by-step procedures

Benjamin M. Delory; Emanuela W.A. Weidlich; Richard van Duijnen; Loïc Pagès; Vicky M. Temperton

doi:10.1007/978-1-4939-7747-5_1

Measuring plant root traits under controlled and field conditions: Step-by-step procedures

Benjamin M. Delory^*, Emanuela W.A. Weidlich, Richard van Duijnen, Loïc Pagès, Vicky M. Temperton

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.

Original language	English
Title of host publication	Root Development
Subtitle of host publication	Methods and Protocols
Editors	Daniela Ristova, Elke Barbez
Place of Publication	New York
Publisher	Humana Press
Pages	3-22
Number of pages	20
Edition	1
ISBN (Electronic)	978-1-4939-7747-5
ISBN (Print)	978-1-4939-7746-8
DOIs	https://doi.org/10.1007/978-1-4939-7747-5_1
Publication status	Published - 11 Mar 2018
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana New York
Volume	1761
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

Funding

We would like to thank Dr. Thomas Niemeyer (Leuphana University, Germany) for its excellent technical support. We are also thankful to Dr. Guillaume Lobet for providing some images used in this book chapter. This research was funded by CNPq Brazil (Ciência Sem Fronteiras Program) with the PhD scholarship of Emanuela W.A. Weidlich, BMBF in Germany for the INPLAMINT PhD project of Richard van Duijnen within the BonaRes soils programme, as well as by the Chair of Ecosystem Functioning and Services, Leuphana University, Lüneburg, Germany.

Funders	Funder number
Bundesministerium für Bildung und Forschung
Conselho Nacional de Desenvolvimento Científico e Tecnológico

Keywords

Image analysis
Ingrowth core method
Phenotyping
Rhizobox
Root staining
Root traits
Root washing
Soil coring

Access to Document

10.1007/978-1-4939-7747-5_1

Cite this

Delory, B. M., Weidlich, E. W. A., van Duijnen, R., Pagès, L., & Temperton, V. M. (2018). Measuring plant root traits under controlled and field conditions: Step-by-step procedures. In D. Ristova, & E. Barbez (Eds.), Root Development: Methods and Protocols (1 ed., pp. 3-22). (Methods in Molecular Biology; Vol. 1761). Humana Press. https://doi.org/10.1007/978-1-4939-7747-5_1

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abstract = "In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.",

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Measuring plant root traits under controlled and field conditions: Step-by-step procedures. / Delory, Benjamin M.; Weidlich, Emanuela W.A.; van Duijnen, Richard et al.
Root Development: Methods and Protocols. ed. / Daniela Ristova; Elke Barbez. 1. ed. New York: Humana Press, 2018. p. 3-22 (Methods in Molecular Biology; Vol. 1761).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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Measuring plant root traits under controlled and field conditions: Step-by-step procedures

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Keywords

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