TY - JOUR
T1 - Measuring endogenous corticosterone in laboratory mice - a mapping review, meta-analysis, and open source database
AU - Van der Mierden, Stevie
AU - Leenaars, Cathalijn H C
AU - Boyle, Erin C
AU - Ripoli, Florenza L
AU - Gass, Peter
AU - Durst, Mattea
AU - Goerlich-Jansson, Vivian C
AU - Jirkof, Paulin
AU - Keubler, Lydia M
AU - Talbot, Steven R
AU - Habedank, Anne
AU - Lewejohann, Lars
AU - Tolba, Rene H
AU - Bleich, André
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2021
Y1 - 2021
N2 - Evaluating stress in laboratory animals is a key principle in animal welfare. Measuring corticosterone is a common method to assess stress in laboratory mice. There are, however, numerous methods to measure glucocorticoids with differences in sample matrix (e.g., plasma, urine) and quantification techniques (e.g., enzyme immunoassay or radioimmunoassay). Here, the authors present a mapping review and a searchable database, giving a complete overview of all studies measuring endogenous corticosterone in mice up to February 2018. For each study, information was recorded regarding mouse strain and sex; corticosterone sample matrix and quantification technique; and whether the study covered the research theme animal welfare, neuroscience, stress, inflammation, or pain (the themes of specific interest in our consortium). Using all database entries for the year 2012, an exploratory meta-regression was performed to determine the effect of predictors on basal corticosterone concentrations. Seventy-five studies were included using the predictors sex, time-since-lights-on, sample matrix, quantification technique, age of the mice, and type of control. Sex, time-since-lights-on, and type of control significantly affected basal corticosterone concentrations. The resulting database can be used, inter alia, for preventing unnecessary duplication of experiments, identifying knowledge gaps, and standardizing or heterogenizing methodologies. These results will help plan more efficient and valid experiments in the future and can answer new questions in silico using meta-analyses.
AB - Evaluating stress in laboratory animals is a key principle in animal welfare. Measuring corticosterone is a common method to assess stress in laboratory mice. There are, however, numerous methods to measure glucocorticoids with differences in sample matrix (e.g., plasma, urine) and quantification techniques (e.g., enzyme immunoassay or radioimmunoassay). Here, the authors present a mapping review and a searchable database, giving a complete overview of all studies measuring endogenous corticosterone in mice up to February 2018. For each study, information was recorded regarding mouse strain and sex; corticosterone sample matrix and quantification technique; and whether the study covered the research theme animal welfare, neuroscience, stress, inflammation, or pain (the themes of specific interest in our consortium). Using all database entries for the year 2012, an exploratory meta-regression was performed to determine the effect of predictors on basal corticosterone concentrations. Seventy-five studies were included using the predictors sex, time-since-lights-on, sample matrix, quantification technique, age of the mice, and type of control. Sex, time-since-lights-on, and type of control significantly affected basal corticosterone concentrations. The resulting database can be used, inter alia, for preventing unnecessary duplication of experiments, identifying knowledge gaps, and standardizing or heterogenizing methodologies. These results will help plan more efficient and valid experiments in the future and can answer new questions in silico using meta-analyses.
KW - 3Rs
KW - corticosterone
KW - mapping review
KW - meta-regression
KW - mice
UR - http://www.scopus.com/inward/record.url?scp=85100280084&partnerID=8YFLogxK
U2 - 10.14573/altex.2004221
DO - 10.14573/altex.2004221
M3 - Review article
C2 - 33086382
SN - 1868-596X
VL - 38
SP - 111
EP - 122
JO - Altex
JF - Altex
IS - 1
ER -