Abstract
Antisera for radioimmunoassay (RIA) against testosterone, 11-oxo-testosterone, 11β-hydroxytestosterone, 11-oxo-androstenedione, and 11β-hydroxyandrostenedione were raised in female rabbits. The RIAs were tested for their suitability for quantification of the steroids after thin layer chromatography of ether extracts from plasma pools of immature and maturing male rainbow trout. Results from control experiments of the RIAs (buffer blanks, sensitivity, cross reactivity, intra- and inter-assay coefficients of variation, accuracy) were all within a normal range. These RIAs should be helpful tools to test the significance of the hypothesis concerning the "interrenal-liver-gonad axis" which has been proposed to account for the high androgen levels in male trout during high reproductive activity.
| Original language | English |
|---|---|
| Pages (from-to) | 717-726 |
| Number of pages | 10 |
| Journal | Steroids |
| Volume | 46 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 1985 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by DFG-grant Bl 62/15-l. The author is indebted to Prof.Dr.R.Reinboth (Johannes Gutenberg Universitat Mainz) for carrying out the recrystallization experiments, to R.Oberstebrink-Scholl and P.Lembke Universitat Bochum) for expert assistance valuable discussion.
Funding
This study was supported by DFG-grant Bl 62/15-l. The author is indebted to Prof.Dr.R.Reinboth (Johannes Gutenberg Universitat Mainz) for carrying out the recrystallization experiments, to R.Oberstebrink-Scholl and P.Lembke Universitat Bochum) for expert assistance valuable discussion.