MDR1 P-glycoprotein is a lipid translocase of broad specifity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine

A. van Helvoort; A.J. Smith; H. Sprong; I. Fritzsche; A.H. Schinkel; P. Borst; G. van Meer

doi:10.1016/S0092-8674(00)81370-7

MDR1 P-glycoprotein is a lipid translocase of broad specifity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine

A. van Helvoort, A.J. Smith, H. Sprong, I. Fritzsche, A.H. Schinkel, P. Borst, G. van Meer

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The human MDR1 P-glycoprotein (Pgp) extrudes a variety of drugs across the plasma membrane. The homologous MDR3 Pgp is required for phosphatidylcholine secretion into bile. After stable transfection of epithelial LLC-PK1 cells, MDR1 and MDR3 Pgp were localized in the apical membrane. At 15 degrees C, newly synthesized short-chain analogs of various membrane lipids were recovered in the apical albumin-containing medium of MDR1 cells but not control cells. MDR inhibitors and energy depletion reduced apical release. MDR3 cells exclusively released a short-chain phosphatidylcholine. Since no vesicular secretion occurs at 15 degrees C, the short-chain lipids must have been translocated by the Pgps across the plasma membrane before extraction into the medium by the lipid-acceptor albumin.

Original language	English
Pages (from-to)	507-517
Number of pages	11
Journal	Cell
Volume	87
Issue number	3
DOIs	https://doi.org/10.1016/S0092-8674(00)81370-7
Publication status	Published - 1996

Keywords

Econometric and Statistical Methods: General
Geneeskunde(GENK)
Bescherming en bevordering van de menselijke gezondheid
Other medical specialities

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title = "MDR1 P-glycoprotein is a lipid translocase of broad specifity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine",

abstract = "The human MDR1 P-glycoprotein (Pgp) extrudes a variety of drugs across the plasma membrane. The homologous MDR3 Pgp is required for phosphatidylcholine secretion into bile. After stable transfection of epithelial LLC-PK1 cells, MDR1 and MDR3 Pgp were localized in the apical membrane. At 15 degrees C, newly synthesized short-chain analogs of various membrane lipids were recovered in the apical albumin-containing medium of MDR1 cells but not control cells. MDR inhibitors and energy depletion reduced apical release. MDR3 cells exclusively released a short-chain phosphatidylcholine. Since no vesicular secretion occurs at 15 degrees C, the short-chain lipids must have been translocated by the Pgps across the plasma membrane before extraction into the medium by the lipid-acceptor albumin.",

keywords = "Econometric and Statistical Methods: General, Geneeskunde(GENK), Bescherming en bevordering van de menselijke gezondheid, Other medical specialities",

author = "{van Helvoort}, A. and A.J. Smith and H. Sprong and I. Fritzsche and A.H. Schinkel and P. Borst and {van Meer}, G.",

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T1 - MDR1 P-glycoprotein is a lipid translocase of broad specifity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine

AU - van Helvoort, A.

AU - Smith, A.J.

AU - Sprong, H.

AU - Fritzsche, I.

AU - Schinkel, A.H.

AU - Borst, P.

AU - van Meer, G.

N1 - keypublication

PY - 1996

Y1 - 1996

N2 - The human MDR1 P-glycoprotein (Pgp) extrudes a variety of drugs across the plasma membrane. The homologous MDR3 Pgp is required for phosphatidylcholine secretion into bile. After stable transfection of epithelial LLC-PK1 cells, MDR1 and MDR3 Pgp were localized in the apical membrane. At 15 degrees C, newly synthesized short-chain analogs of various membrane lipids were recovered in the apical albumin-containing medium of MDR1 cells but not control cells. MDR inhibitors and energy depletion reduced apical release. MDR3 cells exclusively released a short-chain phosphatidylcholine. Since no vesicular secretion occurs at 15 degrees C, the short-chain lipids must have been translocated by the Pgps across the plasma membrane before extraction into the medium by the lipid-acceptor albumin.

AB - The human MDR1 P-glycoprotein (Pgp) extrudes a variety of drugs across the plasma membrane. The homologous MDR3 Pgp is required for phosphatidylcholine secretion into bile. After stable transfection of epithelial LLC-PK1 cells, MDR1 and MDR3 Pgp were localized in the apical membrane. At 15 degrees C, newly synthesized short-chain analogs of various membrane lipids were recovered in the apical albumin-containing medium of MDR1 cells but not control cells. MDR inhibitors and energy depletion reduced apical release. MDR3 cells exclusively released a short-chain phosphatidylcholine. Since no vesicular secretion occurs at 15 degrees C, the short-chain lipids must have been translocated by the Pgps across the plasma membrane before extraction into the medium by the lipid-acceptor albumin.

KW - Econometric and Statistical Methods: General

KW - Geneeskunde(GENK)

KW - Bescherming en bevordering van de menselijke gezondheid

KW - Other medical specialities

U2 - 10.1016/S0092-8674(00)81370-7

DO - 10.1016/S0092-8674(00)81370-7

M3 - Article

SN - 0092-8674

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SP - 507

EP - 517

JO - Cell

JF - Cell

IS - 3

ER -

MDR1 P-glycoprotein is a lipid translocase of broad specifity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine

Abstract

Keywords

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