Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared

E. Nordhoff; A. Ingendoh; R. Cramer; A. Overberg; B. Stahl; M. Karas; F. Hillenkamp; P. F. Crain; B. Chait

doi:10.1002/rcm.1290061212

Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared

E. Nordhoff^*
, A. Ingendoh
, R. Cramer
, A. Overberg
, B. Stahl
, M. Karas
, F. Hillenkamp
, P. F. Crain
, B. Chait

^*Corresponding author for this work

University of Münster
University of Utah
Rockefeller University
extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A number of different matrices have been tested and compared for ultraviolet and ubfrared (UV and IR) matrix‐assisted lasedr desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from Ecoli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR‐MALDI‐MS was determined.

Original language	English
Pages (from-to)	771-776
Number of pages	6
Journal	Rapid Communications in Mass Spectrometry
Volume	6
Issue number	12
DOIs	https://doi.org/10.1002/rcm.1290061212
Publication status	Published - Dec 1992
Externally published	Yes

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title = "Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared",

abstract = "A number of different matrices have been tested and compared for ultraviolet and ubfrared (UV and IR) matrix‐assisted lasedr desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from Ecoli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR‐MALDI‐MS was determined.",

author = "E. Nordhoff and A. Ingendoh and R. Cramer and A. Overberg and B. Stahl and M. Karas and F. Hillenkamp and Crain, \{P. F.\} and B. Chait",

year = "1992",

month = dec,

doi = "10.1002/rcm.1290061212",

language = "English",

volume = "6",

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TY - JOUR

T1 - Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared

AU - Nordhoff, E.

AU - Ingendoh, A.

AU - Cramer, R.

AU - Overberg, A.

AU - Stahl, B.

AU - Karas, M.

AU - Hillenkamp, F.

AU - Crain, P. F.

AU - Chait, B.

PY - 1992/12

Y1 - 1992/12

N2 - A number of different matrices have been tested and compared for ultraviolet and ubfrared (UV and IR) matrix‐assisted lasedr desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from Ecoli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR‐MALDI‐MS was determined.

AB - A number of different matrices have been tested and compared for ultraviolet and ubfrared (UV and IR) matrix‐assisted lasedr desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from Ecoli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR‐MALDI‐MS was determined.

UR - https://www.scopus.com/pages/publications/0027031549

U2 - 10.1002/rcm.1290061212

DO - 10.1002/rcm.1290061212

M3 - Article

C2 - 1283705

AN - SCOPUS:0027031549

SN - 0951-4198

VL - 6

SP - 771

EP - 776

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 12

ER -

Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared

Abstract

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