Abstract
Matrix-assisted laser desorption/ionization mass spectrometry
imaging (MALDI-MSI) has established itself among the plethora of mass
spectrometry applications. In the biomedical field, MALDI-MSI is being
more frequently recognized as a new method for the discovery of biomarkers
and targets of treatment, classification of diseased and healthy tissues, or
prediction of the outcome of a pathology. The technology has been used to
study the localization of proteolytic peptides directly on tissue sections. A
direct correlation between the detected peptides and the distribution and
identity of the original precursor protein is the ultimate goal of any MALDIMSI
experiment. Enzymatic digestion protocols are commonly used to reveal
the protein signature of these complex tissues. Considerations that pertain to
methods of sample preparation, on-tissue digestion, data analysis, and
visualization will be addressed. This review will also discuss selected
applications of on-tissue digestion combined with the MALDI-MSI technology in biomedicine.
Original language | English |
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Journal | Journal of Proteome Research |
DOIs | |
Publication status | Published - 2013 |