Mass spectrometric investigations of human milk oligosaccharides

Anja Pfenninger; M. Karas; B. Finke; B. Stahl; G. Sawatzki

doi:10.1007/978-1-4615-1371-1_35

Mass spectrometric investigations of human milk oligosaccharides

Anja Pfenninger^*, M. Karas, B. Finke, B. Stahl, G. Sawatzki

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.

Original language	English
Pages (from-to)	279-284
Number of pages	6
Journal	Advances in Experimental Medicine and Biology
Volume	501
DOIs	https://doi.org/10.1007/978-1-4615-1371-1_35
Publication status	Published - 2001
Externally published	Yes

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title = "Mass spectrometric investigations of human milk oligosaccharides",

abstract = "Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.",

author = "Anja Pfenninger and M. Karas and B. Finke and B. Stahl and G. Sawatzki",

year = "2001",

doi = "10.1007/978-1-4615-1371-1_35",

language = "English",

volume = "501",

pages = "279--284",

journal = "Advances in Experimental Medicine and Biology",

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T1 - Mass spectrometric investigations of human milk oligosaccharides

AU - Pfenninger, Anja

AU - Karas, M.

AU - Finke, B.

AU - Stahl, B.

AU - Sawatzki, G.

PY - 2001

Y1 - 2001

N2 - Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.

AB - Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.

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DO - 10.1007/978-1-4615-1371-1_35

M3 - Article

C2 - 11787691

AN - SCOPUS:0035703578

SN - 0065-2598

VL - 501

SP - 279

EP - 284

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Mass spectrometric investigations of human milk oligosaccharides

Abstract

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