Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk

Anja Pfenninger; Shiu Yung Chan; Michael Karas; Berndt Finke; Bernd Stahl; Catherine E. Costello

doi:10.1016/j.ijms.2008.09.005

Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk

Anja Pfenninger, Shiu Yung Chan, Michael Karas, Berndt Finke, Bernd Stahl, Catherine E. Costello^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Complex mixtures of high-molecular weight fractions of pooled neutral human milk oligosaccharides (obtained via gel permeation chromatography) have been investigated. The subfractions were each permethylated and analyzed by high-resolution mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, in order to investigate their oligosaccharide compositions. The obtained spectra reveal that human milk contains more complex neutral oligosaccharides than have been described previously; the data show that these oligosaccharides can be highly fucosylated, and that their poly-N-acetyllactosamine cores are substituted with up to 10 fucose residues on an oligosaccharide that has 7-N-acetyllactosamine units. This is the first report of the existence in human milk of this large range of highly fucosylated oligosaccharides which possess novel, potentially immunologically active structures.

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	International Journal of Mass Spectrometry
Volume	278
Issue number	2-3
DOIs	https://doi.org/10.1016/j.ijms.2008.09.005
Publication status	Published - 1 Dec 2008
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank the Deutsche Akademische Austauschdienst (DAAD) for providing Anja Pfenninger generous travel grants. Financial support from the Bundesministerium für Bildung und Forschung (BMBF) under Grant BEO/22 0311229 (to MK) and from the US PHS Grant No. P41 RR10888 (to CEC) is also gratefully acknowledged. N. Viseux, E. Mirgorodskaya and P.B. O’Connor contributed helpful insight on FTICR MS experimental procedures.

Funding

The authors thank the Deutsche Akademische Austauschdienst (DAAD) for providing Anja Pfenninger generous travel grants. Financial support from the Bundesministerium für Bildung und Forschung (BMBF) under Grant BEO/22 0311229 (to MK) and from the US PHS Grant No. P41 RR10888 (to CEC) is also gratefully acknowledged. N. Viseux, E. Mirgorodskaya and P.B. O’Connor contributed helpful insight on FTICR MS experimental procedures.

Keywords

Carbohydrates
FTICR MS
Fucosylation
MALDI
Mixture analysis

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10.1016/j.ijms.2008.09.005

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title = "Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk",

abstract = "Complex mixtures of high-molecular weight fractions of pooled neutral human milk oligosaccharides (obtained via gel permeation chromatography) have been investigated. The subfractions were each permethylated and analyzed by high-resolution mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, in order to investigate their oligosaccharide compositions. The obtained spectra reveal that human milk contains more complex neutral oligosaccharides than have been described previously; the data show that these oligosaccharides can be highly fucosylated, and that their poly-N-acetyllactosamine cores are substituted with up to 10 fucose residues on an oligosaccharide that has 7-N-acetyllactosamine units. This is the first report of the existence in human milk of this large range of highly fucosylated oligosaccharides which possess novel, potentially immunologically active structures.",

keywords = "Carbohydrates, FTICR MS, Fucosylation, MALDI, Mixture analysis",

author = "Anja Pfenninger and Chan, {Shiu Yung} and Michael Karas and Berndt Finke and Bernd Stahl and Costello, {Catherine E.}",

note = "Funding Information: The authors thank the Deutsche Akademische Austauschdienst (DAAD) for providing Anja Pfenninger generous travel grants. Financial support from the Bundesministerium f{\"u}r Bildung und Forschung (BMBF) under Grant BEO/22 0311229 (to MK) and from the US PHS Grant No. P41 RR10888 (to CEC) is also gratefully acknowledged. N. Viseux, E. Mirgorodskaya and P.B. O{\textquoteright}Connor contributed helpful insight on FTICR MS experimental procedures.",

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doi = "10.1016/j.ijms.2008.09.005",

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AU - Pfenninger, Anja

AU - Chan, Shiu Yung

AU - Karas, Michael

AU - Finke, Berndt

AU - Stahl, Bernd

AU - Costello, Catherine E.

N1 - Funding Information: The authors thank the Deutsche Akademische Austauschdienst (DAAD) for providing Anja Pfenninger generous travel grants. Financial support from the Bundesministerium für Bildung und Forschung (BMBF) under Grant BEO/22 0311229 (to MK) and from the US PHS Grant No. P41 RR10888 (to CEC) is also gratefully acknowledged. N. Viseux, E. Mirgorodskaya and P.B. O’Connor contributed helpful insight on FTICR MS experimental procedures.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Complex mixtures of high-molecular weight fractions of pooled neutral human milk oligosaccharides (obtained via gel permeation chromatography) have been investigated. The subfractions were each permethylated and analyzed by high-resolution mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, in order to investigate their oligosaccharide compositions. The obtained spectra reveal that human milk contains more complex neutral oligosaccharides than have been described previously; the data show that these oligosaccharides can be highly fucosylated, and that their poly-N-acetyllactosamine cores are substituted with up to 10 fucose residues on an oligosaccharide that has 7-N-acetyllactosamine units. This is the first report of the existence in human milk of this large range of highly fucosylated oligosaccharides which possess novel, potentially immunologically active structures.

AB - Complex mixtures of high-molecular weight fractions of pooled neutral human milk oligosaccharides (obtained via gel permeation chromatography) have been investigated. The subfractions were each permethylated and analyzed by high-resolution mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, in order to investigate their oligosaccharide compositions. The obtained spectra reveal that human milk contains more complex neutral oligosaccharides than have been described previously; the data show that these oligosaccharides can be highly fucosylated, and that their poly-N-acetyllactosamine cores are substituted with up to 10 fucose residues on an oligosaccharide that has 7-N-acetyllactosamine units. This is the first report of the existence in human milk of this large range of highly fucosylated oligosaccharides which possess novel, potentially immunologically active structures.

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ER -

Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk

Abstract

Bibliographical note

Funding

Keywords

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