Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

Bas W M van Balkom; Renske A van Gestel; Jos F H M Brouwers; Jeroen Krijgsveld; Aloysius G M Tielens; Albert J R Heck; Jaap J van Hellemond

doi:10.1021/pr050036w

Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

Bas W M van Balkom
, Renske A van Gestel
, Jos F H M Brouwers
, Jeroen Krijgsveld
, Aloysius G M Tielens
, Albert J R Heck
, Jaap J van Hellemond

Sub Biomolecular Mass Spectrometry and Proteomics

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.

Original language	English
Pages (from-to)	958-966
Number of pages	9
Journal	Journal of Proteome Research
Volume	4
Issue number	3
DOIs	https://doi.org/10.1021/pr050036w
Publication status	Published - 15 Jun 2005

Keywords

Animals
Chromatography, High Pressure Liquid
Computational Biology
Electrophoresis, Polyacrylamide Gel
Helminth Proteins
Mass Spectrometry
Proteome
Proteomics
Schistosoma mansoni

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10.1021/pr050036w

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title = "Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome",

abstract = "Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.",

keywords = "Animals, Chromatography, High Pressure Liquid, Computational Biology, Electrophoresis, Polyacrylamide Gel, Helminth Proteins, Mass Spectrometry, Proteome, Proteomics, Schistosoma mansoni",

author = "\{van Balkom\}, \{Bas W M\} and \{van Gestel\}, \{Renske A\} and Brouwers, \{Jos F H M\} and Jeroen Krijgsveld and Tielens, \{Aloysius G M\} and Heck, \{Albert J R\} and \{van Hellemond\}, \{Jaap J\}",

year = "2005",

month = jun,

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doi = "10.1021/pr050036w",

language = "English",

volume = "4",

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journal = "Journal of Proteome Research",

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publisher = "American Chemical Society",

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T1 - Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

AU - van Balkom, Bas W M

AU - van Gestel, Renske A

AU - Brouwers, Jos F H M

AU - Krijgsveld, Jeroen

AU - Tielens, Aloysius G M

AU - Heck, Albert J R

AU - van Hellemond, Jaap J

PY - 2005/6/15

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N2 - Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.

AB - Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.

KW - Animals

KW - Chromatography, High Pressure Liquid

KW - Computational Biology

KW - Electrophoresis, Polyacrylamide Gel

KW - Helminth Proteins

KW - Mass Spectrometry

KW - Proteome

KW - Proteomics

KW - Schistosoma mansoni

U2 - 10.1021/pr050036w

DO - 10.1021/pr050036w

M3 - Article

C2 - 15952743

SN - 1535-3893

VL - 4

SP - 958

EP - 966

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 3

ER -

Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

Abstract

Keywords

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