Mapping of the Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis

L Fontanesi, R Davoli, C Zijlstra, A A Bosma, V Russo

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Two expressed sequence tags were isolated from a porcine skeletal muscle cDNA library and identified as the putative partial cDNAs of the porcine Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes after sequencing and homology search. Results of analysis of a pig-rodent somatic cell hybrid panel by PCR allowed the assignments of ATP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5.

Original languageEnglish
Pages (from-to)57-9
Number of pages3
JournalAnimal Genetics
Volume30
Issue number1
Publication statusPublished - Feb 1999

Keywords

  • Animals
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1
  • Chromosomes, Human, Pair 12
  • Expressed Sequence Tags
  • Humans
  • Muscle, Skeletal
  • Phosphofructokinase-1
  • Sequence Homology, Nucleic Acid
  • Sodium-Potassium-Exchanging ATPase
  • Swine

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