Mammalian species identification by interspersed repeat PCR fingerprinting

J. B. Buntjer; J. A. Lenstra

doi:10.1038/sj.jim.2900540

Mammalian species identification by interspersed repeat PCR fingerprinting

J. B. Buntjer, J. A. Lenstra^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Most DNA methods for species identification of animal tissues test the presence/absence of one species per assay, requiring several tests for a complete analysis and prior knowledge of the species that are potentially present in the sample. Here we demonstrate that PCR with fluorescently labeled MIR (mammalian-wide interspersed repeat) primers generate fingerprints that are suitable for rapid identification of known and unknown species on an automatic sequencing apparatus and with computer-assisted data processing. The method allows the analysis of processed meat samples and offers a convenient alternative to sequencing of mitochondrial DNA.

Original language	English
Pages (from-to)	121-127
Number of pages	7
Journal	Journal of industrial microbiology & biotechnology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1038/sj.jim.2900540
Publication status	Published - 1 Dec 1998

Keywords

Animal tissues
DNA fingerprinting
PCR
Species identification

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10.1038/sj.jim.2900540

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year = "1998",

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AB - Most DNA methods for species identification of animal tissues test the presence/absence of one species per assay, requiring several tests for a complete analysis and prior knowledge of the species that are potentially present in the sample. Here we demonstrate that PCR with fluorescently labeled MIR (mammalian-wide interspersed repeat) primers generate fingerprints that are suitable for rapid identification of known and unknown species on an automatic sequencing apparatus and with computer-assisted data processing. The method allows the analysis of processed meat samples and offers a convenient alternative to sequencing of mitochondrial DNA.

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Mammalian species identification by interspersed repeat PCR fingerprinting

Abstract

Keywords

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