TY - JOUR
T1 - Luminescence thermometry for: In situ temperature measurements in microfluidic devices
AU - Geitenbeek, Robin G.
AU - Vollenbroek, Jeroen C.
AU - Weijgertze, Hannah M.H.
AU - Tregouet, Corentin B.M.
AU - Nieuwelink, Anne Eva
AU - Kennedy, Chris L.
AU - Weckhuysen, Bert M.
AU - Lohse, Detlef
AU - Van Blaaderen, Alfons
AU - Van Den Berg, Albert
AU - Odijk, Mathieu
AU - Meijerink, Andries
PY - 2019/2/25
Y1 - 2019/2/25
N2 - Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.
AB - Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er 3+ in NaYF 4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.
U2 - 10.1039/c8lc01292j
DO - 10.1039/c8lc01292j
M3 - Article
SN - 1473-0197
VL - 19
SP - 1236
EP - 1246
JO - Lab on a Chip
JF - Lab on a Chip
IS - 7
ER -