Low Seroprevalence of Brucellosis in Humans and Small Ruminants in the Gambia

Eveline A Germeraad; Lenny Hogerwerf; Tisbeh Faye-Joof; Bart Goossens; Wim van der Hoek; Momodou Jeng; Modou Lamin; Ismaila L Manneh; Davis Nwakanma; Hendrik I J Roest; Arss Secka; Arjan Stegeman; Rita Wegmüller; Marianne A B van der Sande; Ousman Secka

doi:10.1371/journal.pone.0166035

Low Seroprevalence of Brucellosis in Humans and Small Ruminants in the Gambia

Eveline A Germeraad, Lenny Hogerwerf, Tisbeh Faye-Joof, Bart Goossens, Wim van der Hoek, Momodou Jeng, Modou Lamin, Ismaila L Manneh, Davis Nwakanma, Hendrik I J Roest, Arss Secka, Arjan Stegeman, Rita Wegmüller, Marianne A B van der Sande, Ousman Secka

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Brucellosis is a worldwide zoonosis with significant impact on rural livelihoods and a potentially underestimated contributor to febrile illnesses. The aim of this study was to estimate the seroprevalence of brucellosis in humans and small ruminants in The Gambia.

METHODS: The study was carried out in rural and urban areas. In 12 rural villages in Kiang West district, sera were collected from humans (n = 599) and small ruminants (n = 623) from the same compounds. From lactating small ruminants, milk samples and vaginal swabs were obtained. At the urban study sites, sera were collected from small ruminants (n = 500) from slaughterhouses and livestock markets. Information on possible risk factors for seropositivity was collected through questionnaires. Sera were screened for antibodies against Brucella spp. with the Rose Bengal Test, ELISA and Micro Agglutination Test (human sera only). PCR was performed on 10 percent of the milk samples and vaginal swabs from small ruminants.

RESULTS: One human and 14 sheep sera were positive by the Rose Bengal Test. The rest were negative in all serological tests used. The PCR results were all negative.

CONCLUSIONS: The results suggest that brucellosis is currently not a generalized problem in humans or small ruminants in The Gambia.

Original language	English
Article number	e0166035
Journal	PLoS One
Volume	11
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0166035
Publication status	Published - 8 Nov 2016

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Germeraad, E. A., Hogerwerf, L., Faye-Joof, T., Goossens, B., van der Hoek, W., Jeng, M., Lamin, M., Manneh, I. L., Nwakanma, D., Roest, H. I. J., Secka, A., Stegeman, A., Wegmüller, R., van der Sande, M. A. B., & Secka, O. (2016). Low Seroprevalence of Brucellosis in Humans and Small Ruminants in the Gambia. PLoS One, 11(11), Article e0166035. https://doi.org/10.1371/journal.pone.0166035

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abstract = "BACKGROUND: Brucellosis is a worldwide zoonosis with significant impact on rural livelihoods and a potentially underestimated contributor to febrile illnesses. The aim of this study was to estimate the seroprevalence of brucellosis in humans and small ruminants in The Gambia.METHODS: The study was carried out in rural and urban areas. In 12 rural villages in Kiang West district, sera were collected from humans (n = 599) and small ruminants (n = 623) from the same compounds. From lactating small ruminants, milk samples and vaginal swabs were obtained. At the urban study sites, sera were collected from small ruminants (n = 500) from slaughterhouses and livestock markets. Information on possible risk factors for seropositivity was collected through questionnaires. Sera were screened for antibodies against Brucella spp. with the Rose Bengal Test, ELISA and Micro Agglutination Test (human sera only). PCR was performed on 10 percent of the milk samples and vaginal swabs from small ruminants.RESULTS: One human and 14 sheep sera were positive by the Rose Bengal Test. The rest were negative in all serological tests used. The PCR results were all negative.CONCLUSIONS: The results suggest that brucellosis is currently not a generalized problem in humans or small ruminants in The Gambia.",

author = "Germeraad, {Eveline A} and Lenny Hogerwerf and Tisbeh Faye-Joof and Bart Goossens and {van der Hoek}, Wim and Momodou Jeng and Modou Lamin and Manneh, {Ismaila L} and Davis Nwakanma and Roest, {Hendrik I J} and Arss Secka and Arjan Stegeman and Rita Wegm{\"u}ller and {van der Sande}, {Marianne A B} and Ousman Secka",

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doi = "10.1371/journal.pone.0166035",

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AU - Germeraad, Eveline A

AU - Hogerwerf, Lenny

AU - Faye-Joof, Tisbeh

AU - Goossens, Bart

AU - van der Hoek, Wim

AU - Jeng, Momodou

AU - Lamin, Modou

AU - Manneh, Ismaila L

AU - Nwakanma, Davis

AU - Roest, Hendrik I J

AU - Secka, Arss

AU - Stegeman, Arjan

AU - Wegmüller, Rita

AU - van der Sande, Marianne A B

AU - Secka, Ousman

PY - 2016/11/8

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N2 - BACKGROUND: Brucellosis is a worldwide zoonosis with significant impact on rural livelihoods and a potentially underestimated contributor to febrile illnesses. The aim of this study was to estimate the seroprevalence of brucellosis in humans and small ruminants in The Gambia.METHODS: The study was carried out in rural and urban areas. In 12 rural villages in Kiang West district, sera were collected from humans (n = 599) and small ruminants (n = 623) from the same compounds. From lactating small ruminants, milk samples and vaginal swabs were obtained. At the urban study sites, sera were collected from small ruminants (n = 500) from slaughterhouses and livestock markets. Information on possible risk factors for seropositivity was collected through questionnaires. Sera were screened for antibodies against Brucella spp. with the Rose Bengal Test, ELISA and Micro Agglutination Test (human sera only). PCR was performed on 10 percent of the milk samples and vaginal swabs from small ruminants.RESULTS: One human and 14 sheep sera were positive by the Rose Bengal Test. The rest were negative in all serological tests used. The PCR results were all negative.CONCLUSIONS: The results suggest that brucellosis is currently not a generalized problem in humans or small ruminants in The Gambia.

AB - BACKGROUND: Brucellosis is a worldwide zoonosis with significant impact on rural livelihoods and a potentially underestimated contributor to febrile illnesses. The aim of this study was to estimate the seroprevalence of brucellosis in humans and small ruminants in The Gambia.METHODS: The study was carried out in rural and urban areas. In 12 rural villages in Kiang West district, sera were collected from humans (n = 599) and small ruminants (n = 623) from the same compounds. From lactating small ruminants, milk samples and vaginal swabs were obtained. At the urban study sites, sera were collected from small ruminants (n = 500) from slaughterhouses and livestock markets. Information on possible risk factors for seropositivity was collected through questionnaires. Sera were screened for antibodies against Brucella spp. with the Rose Bengal Test, ELISA and Micro Agglutination Test (human sera only). PCR was performed on 10 percent of the milk samples and vaginal swabs from small ruminants.RESULTS: One human and 14 sheep sera were positive by the Rose Bengal Test. The rest were negative in all serological tests used. The PCR results were all negative.CONCLUSIONS: The results suggest that brucellosis is currently not a generalized problem in humans or small ruminants in The Gambia.

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DO - 10.1371/journal.pone.0166035

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JO - PLoS One

JF - PLoS One

IS - 11

M1 - e0166035

ER -

Low Seroprevalence of Brucellosis in Humans and Small Ruminants in the Gambia

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