Abstract
Since September 2023, bluetongue virus serotype 3 has been circulating in the Netherlands. Cattle can exhibit a range of clinical signs from sub-clinical to severe, but they are also known to play an important role as a virus reservoir in bluetongue epidemics. We closely monitored an outbreak on a commercial dairy herd by daily clinical inspection, sensor data and longitudinal biweekly real-time PCR testing. PCR testing was performed on 18 cattle from the start of the bluetongue virus serotype 3 outbreak until two consecutive blood samples tested negative, 24–28 weeks after the start of the monitoring. In conclusion, this case study has shown that sensor data can contribute to detecting bluetongue virus serotype 3-infected cattle. Furthermore, it appeared that the majority of cattle remained positive by real-time PCR for approximately 6 months from the start of the infection.
| Original language | English |
|---|---|
| Article number | e70068 |
| Journal | Veterinary Record Case Reports |
| Volume | 13 |
| Issue number | 2 |
| Early online date | 8 Apr 2025 |
| DOIs | |
| Publication status | Published - Jun 2025 |
Bibliographical note
Publisher Copyright:© 2025 The Author(s). Veterinary Record Case Reports published by John Wiley & Sons Ltd on behalf of British Veterinary Association.
Funding
We want to acknowledge the staff of the Tolakker farm and many students for facilitating this study. We would like to thank the diagnostic and crisis organization (DCO) department at WBVR for processing the samples. This study was financially supported by ZuivelNL and the Dutch Ministry of Agriculture, Fishery, Food Safety and Nature under project WOT-01-002-041 VZVD.
| Funders |
|---|
| Dutch Ministry of Agriculture |
| ZuivelNL |
| Dutch Ministry of Agriculture, Fishery, Food Safety and Nature |
Keywords
- bluetongue
- clinical practice
- dairy cattle
- disease investigation
- RT-PCR techniques