Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism

Sofie J J Marynissen; Pascale M Y Smets; Liesbeth F E Ghys; Dominique Paepe; Joris Delanghe; Sara Galac; Evelyne Meyer; Hervé P Lefebvre; Sylvie Daminet

doi:10.1111/vcp.12365

Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism

Sofie J J Marynissen, Pascale M Y Smets, Liesbeth F E Ghys, Dominique Paepe, Joris Delanghe, Sara Galac, Evelyne Meyer, Hervé P Lefebvre, Sylvie Daminet

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Serum cystatin C (sCysC) is used as biomarker for glomerular filtration rate (GFR). The effects of diabetes mellitus (DM) on renal function in dogs are unclear. Some renal variables have been evaluated in dogs with hyperadrenocorticism (HAC), but not sCysC.

OBJECTIVES: The purpose of this study was the validation of a particle-enhanced nephelometric immunoassay (PENIA) for measuring canine sCysC, and to assess renal function in dogs with DM or HAC.

METHODS: A PENIA was analytically validated for canine sCysC by determining imprecision and linearity. In a longitudinal 6-month study, renal function of 14 DM dogs was assessed, using serum creatinine, GFR, urinary protein-to-creatinine (UPC) ratio, urinary markers, systolic blood pressure (SBP), and sCysC, and compared to 17 healthy dogs at baseline. Furthermore, sCysC was measured at initial presentation and during a 12-month follow-up in 22 HAC dogs.

RESULTS: The sCysC intra- and inter-assay variation coefficients were < 8% and highly linear (r = .997). About 33% and 67% of DM dogs had persistent proteinuria and systemic hypertension, respectively, but there were no significant differences in GFR, UPC, and urinary markers over time, and compared with healthy dogs at initial presentation. Serum CysC decreased significantly (P < .05) over time within the DM group. It did not change significantly over time within the HAC group.

CONCLUSIONS: A PENIA measured sCysC linearly and precisely. There were no clinically relevant renal alterations over time in dogs with DM, although persistent proteinuria was observed. In dogs with HAC, sCysC measurement was not useful, although significant GFR changes occurred over time.

Original language	English
Pages (from-to)	320–329
Journal	Veterinary clinical pathology / American Society for Veterinary Clinical Pathology
Volume	45
Issue number	2
DOIs	https://doi.org/10.1111/vcp.12365
Publication status	Published - 2016

Keywords

Biomarker; canine; GFR ; kidney; proteinuria

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10.1111/vcp.12365

MarynissenFinal published version, 101 KBLicence: Taverne

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Marynissen, S. J. J., Smets, P. M. Y., Ghys, L. F. E., Paepe, D., Delanghe, J., Galac, S., Meyer, E., Lefebvre, H. P., & Daminet, S. (2016). Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism. Veterinary clinical pathology / American Society for Veterinary Clinical Pathology, 45(2), 320–329. https://doi.org/10.1111/vcp.12365

@article{d84d4976e4fc40019bfa4f83f4c2ce54,

title = "Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism",

abstract = "BACKGROUND: Serum cystatin C (sCysC) is used as biomarker for glomerular filtration rate (GFR). The effects of diabetes mellitus (DM) on renal function in dogs are unclear. Some renal variables have been evaluated in dogs with hyperadrenocorticism (HAC), but not sCysC.OBJECTIVES: The purpose of this study was the validation of a particle-enhanced nephelometric immunoassay (PENIA) for measuring canine sCysC, and to assess renal function in dogs with DM or HAC.METHODS: A PENIA was analytically validated for canine sCysC by determining imprecision and linearity. In a longitudinal 6-month study, renal function of 14 DM dogs was assessed, using serum creatinine, GFR, urinary protein-to-creatinine (UPC) ratio, urinary markers, systolic blood pressure (SBP), and sCysC, and compared to 17 healthy dogs at baseline. Furthermore, sCysC was measured at initial presentation and during a 12-month follow-up in 22 HAC dogs.RESULTS: The sCysC intra- and inter-assay variation coefficients were < 8% and highly linear (r = .997). About 33% and 67% of DM dogs had persistent proteinuria and systemic hypertension, respectively, but there were no significant differences in GFR, UPC, and urinary markers over time, and compared with healthy dogs at initial presentation. Serum CysC decreased significantly (P < .05) over time within the DM group. It did not change significantly over time within the HAC group.CONCLUSIONS: A PENIA measured sCysC linearly and precisely. There were no clinically relevant renal alterations over time in dogs with DM, although persistent proteinuria was observed. In dogs with HAC, sCysC measurement was not useful, although significant GFR changes occurred over time.",

keywords = "Biomarker; canine; GFR ; kidney; proteinuria",

author = "Marynissen, {Sofie J J} and Smets, {Pascale M Y} and Ghys, {Liesbeth F E} and Dominique Paepe and Joris Delanghe and Sara Galac and Evelyne Meyer and Lefebvre, {Herv{\'e} P} and Sylvie Daminet",

note = "{\textcopyright} 2016 American Society for Veterinary Clinical Pathology.",

year = "2016",

doi = "10.1111/vcp.12365",

language = "English",

volume = "45",

pages = " 320–329",

journal = "Veterinary clinical pathology / American Society for Veterinary Clinical Pathology",

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number = "2",

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Marynissen, SJJ, Smets, PMY, Ghys, LFE, Paepe, D, Delanghe, J, Galac, S, Meyer, E, Lefebvre, HP & Daminet, S 2016, 'Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism', Veterinary clinical pathology / American Society for Veterinary Clinical Pathology, vol. 45, no. 2, pp. 320–329. https://doi.org/10.1111/vcp.12365

Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism. / Marynissen, Sofie J J; Smets, Pascale M Y; Ghys, Liesbeth F E et al.
In: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology, Vol. 45, No. 2, 2016, p. 320–329.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism

AU - Marynissen, Sofie J J

AU - Smets, Pascale M Y

AU - Ghys, Liesbeth F E

AU - Paepe, Dominique

AU - Delanghe, Joris

AU - Galac, Sara

AU - Meyer, Evelyne

AU - Lefebvre, Hervé P

AU - Daminet, Sylvie

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Serum cystatin C (sCysC) is used as biomarker for glomerular filtration rate (GFR). The effects of diabetes mellitus (DM) on renal function in dogs are unclear. Some renal variables have been evaluated in dogs with hyperadrenocorticism (HAC), but not sCysC.OBJECTIVES: The purpose of this study was the validation of a particle-enhanced nephelometric immunoassay (PENIA) for measuring canine sCysC, and to assess renal function in dogs with DM or HAC.METHODS: A PENIA was analytically validated for canine sCysC by determining imprecision and linearity. In a longitudinal 6-month study, renal function of 14 DM dogs was assessed, using serum creatinine, GFR, urinary protein-to-creatinine (UPC) ratio, urinary markers, systolic blood pressure (SBP), and sCysC, and compared to 17 healthy dogs at baseline. Furthermore, sCysC was measured at initial presentation and during a 12-month follow-up in 22 HAC dogs.RESULTS: The sCysC intra- and inter-assay variation coefficients were < 8% and highly linear (r = .997). About 33% and 67% of DM dogs had persistent proteinuria and systemic hypertension, respectively, but there were no significant differences in GFR, UPC, and urinary markers over time, and compared with healthy dogs at initial presentation. Serum CysC decreased significantly (P < .05) over time within the DM group. It did not change significantly over time within the HAC group.CONCLUSIONS: A PENIA measured sCysC linearly and precisely. There were no clinically relevant renal alterations over time in dogs with DM, although persistent proteinuria was observed. In dogs with HAC, sCysC measurement was not useful, although significant GFR changes occurred over time.

AB - BACKGROUND: Serum cystatin C (sCysC) is used as biomarker for glomerular filtration rate (GFR). The effects of diabetes mellitus (DM) on renal function in dogs are unclear. Some renal variables have been evaluated in dogs with hyperadrenocorticism (HAC), but not sCysC.OBJECTIVES: The purpose of this study was the validation of a particle-enhanced nephelometric immunoassay (PENIA) for measuring canine sCysC, and to assess renal function in dogs with DM or HAC.METHODS: A PENIA was analytically validated for canine sCysC by determining imprecision and linearity. In a longitudinal 6-month study, renal function of 14 DM dogs was assessed, using serum creatinine, GFR, urinary protein-to-creatinine (UPC) ratio, urinary markers, systolic blood pressure (SBP), and sCysC, and compared to 17 healthy dogs at baseline. Furthermore, sCysC was measured at initial presentation and during a 12-month follow-up in 22 HAC dogs.RESULTS: The sCysC intra- and inter-assay variation coefficients were < 8% and highly linear (r = .997). About 33% and 67% of DM dogs had persistent proteinuria and systemic hypertension, respectively, but there were no significant differences in GFR, UPC, and urinary markers over time, and compared with healthy dogs at initial presentation. Serum CysC decreased significantly (P < .05) over time within the DM group. It did not change significantly over time within the HAC group.CONCLUSIONS: A PENIA measured sCysC linearly and precisely. There were no clinically relevant renal alterations over time in dogs with DM, although persistent proteinuria was observed. In dogs with HAC, sCysC measurement was not useful, although significant GFR changes occurred over time.

KW - Biomarker; canine; GFR ; kidney; proteinuria

U2 - 10.1111/vcp.12365

DO - 10.1111/vcp.12365

M3 - Article

C2 - 27228235

SN - 0275-6382

VL - 45

SP - 320

EP - 329

JO - Veterinary clinical pathology / American Society for Veterinary Clinical Pathology

JF - Veterinary clinical pathology / American Society for Veterinary Clinical Pathology

IS - 2

ER -

Long-term follow-up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism

Abstract

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