Locational memory of macrovessel vascular cells is transcriptionally imprinted

Talitha C F Spanjersberg, Loes A Oosterhoff, Hedwig S Kruitwagen, Noortje A M van den Dungen, Johannes C M Vernooij, Folkert W Asselbergs, Michal Mokry, Bart Spee, Magdalena Harakalova, Frank G van Steenbeek*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems.

Original languageEnglish
Article number13028
Pages (from-to)13028
Number of pages14
JournalScientific Reports
Volume13
Issue number1
DOIs
Publication statusPublished - 10 Aug 2023

Keywords

  • Diversity
  • Epicardium
  • Expression
  • Genes
  • Mesenchyme
  • Mesothelium
  • Models
  • Package
  • Smooth-muscle-cells
  • Tissue

Fingerprint

Dive into the research topics of 'Locational memory of macrovessel vascular cells is transcriptionally imprinted'. Together they form a unique fingerprint.

Cite this