Localization of the pathway of the penicillin biosynthesis in Penicillium chrysogenum

W.H. Muller, T.P. Van Der Krift, A.J.J. Krouwer, H.A.B. Wosten, L. Van Der Voort, E.B. Smaal, A.J. Verkleij

Research output: Contribution to journalArticleAcademicpeer-review


The localization of the enzymes involved in penicillin biosynthesis in penicillium chrysogenum hyphae has been studied by immunological detection methods in combination with electron microscopy and cell fractionation. The results suggest a complicated pathway involving different intracellular locations. The enzyme δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase was found to be associated with membranes or small organelles. The next enzyme isopenicillin N-synthetase appeared to be a cytosolic enzyme. The enzyme which is involved in the last step of penicillin biosynthesis, acyltransferase, was located in organelles with a diameter of 200-800 nm. These organelles, most probably, are microbodies. A positive correlation was found between the capacity for penicillin production and the number of organelles per cell when comparing different P. chrysogenum strains.
Original languageEnglish
Pages (from-to)489-495
Number of pages7
JournalEMBO Journal
Issue number2
Publication statusPublished - 5 Feb 1991


  • compartmentalization
  • microbodies
  • penicillin biosynthesis
  • penicillin G
  • article
  • biosynthesis
  • enzyme localization
  • nonhuman
  • Penicillium chrysogenum
  • priority journal


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