Abstract
Liver organoids are stem cell-derived 3D structures that are generated by liver differentiation signals in the presence of a supporting extracellular matrix. Liver organoids overcome low complexity grade of bidimensional culture and high costs of in vivo models thus representing a turning point for studying liver disease modeling. Liver organoids can be established from different sources as induced pluripotent stem cells (iPSCs), embryonic stem cells (ESCs), hepatoblasts and tissue-derived cells. This novel in vitro system represents an innovative tool to deeper understand the physiology and pathological mechanisms affecting the liver. In this review, we discuss the current advances in the field focusing on their application in modeling diseases, regenerative medicine and drug discovery.
| Original language | English |
|---|---|
| Article number | 835 |
| Pages (from-to) | 1-13 |
| Number of pages | 13 |
| Journal | Biology |
| Volume | 10 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 27 Aug 2021 |
Bibliographical note
Funding Information:This work was supported by the H2020-FETOPEN-2018-2019-2020-01 ENLIGHT, Project number: 964497 and by the COINOR grant STAR Linea1-2018, DOPAncODE, project number: 18-CSP-UNINA-042.Figures were created with https://biorender.com/ (accessed on 1 June 2021).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Funding
This work was supported by the H2020-FETOPEN-2018-2019-2020-01 ENLIGHT, Project number: 964497 and by the COINOR grant STAR Linea1-2018, DOPAncODE, project number: 18-CSP-UNINA-042.Figures were created with https://biorender.com/ (accessed on 1 June 2021).
Keywords
- 3D culture
- Cholangiocytes
- Hepatocyte
- Liver disease modeling
- Personalized medicine
- Stem cells