Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells

DC4Balance consortium; Toni M M van Capel

doi:10.3389/fimmu.2023.1137538

Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells

DC4Balance consortium, Toni M M van Capel

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

INTRODUCTION: Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.

METHODS: Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.

RESULTS: Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.

DISCUSSION: These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.

Original language	English
Article number	1137538
Number of pages	16
Journal	Frontiers in Immunology
Volume	14
DOIs	https://doi.org/10.3389/fimmu.2023.1137538
Publication status	Published - 2023
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Health Holland and “Samenwerkende Gezondheidsfonden” (SGF), with the LSH-TKI project the LSH-TKI project DC4Balance LSHM18056-SGF. The sponsors had no role in the study design, collection, analysis, interpretation of data, report writing, or decision to submit the manuscript for publication. Acknowledgments

Publisher Copyright:
Copyright © 2023 Nagy, Lozano Vigario, Sparrius, van Capel, van Ree, Tas, de Vries, Geijtenbeek, Slütter, de Jong and for the DC4Balance consortium.

Keywords

T regulatory cells (Tregs)
allergies
autoimmunity
dendritic cells
immunotherapy
liposome
tolerance
vitamin D3

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@article{7e471aa159ff42c8b5102606df4783ad,

title = "Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells",

abstract = "INTRODUCTION: Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.METHODS: Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.RESULTS: Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.DISCUSSION: These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.",

keywords = "T regulatory cells (Tregs), allergies, autoimmunity, dendritic cells, immunotherapy, liposome, tolerance, vitamin D3",

author = "{DC4Balance consortium} and Nagy, {No{\'e}mi Anna} and {Lozano Vigario}, Fernando and Rinske Sparrius and {van Capel}, {Toni M M} and {van Ree}, Ronald and Tas, {Sander W} and {de Vries}, {I Jolanda M} and Geijtenbeek, {Teunis B H} and Bram Sl{\"u}tter and {de Jong}, {Esther C}",

note = "Funding Information: This work was supported by Health Holland and “Samenwerkende Gezondheidsfonden” (SGF), with the LSH-TKI project the LSH-TKI project DC4Balance LSHM18056-SGF. The sponsors had no role in the study design, collection, analysis, interpretation of data, report writing, or decision to submit the manuscript for publication. Acknowledgments Publisher Copyright: Copyright {\textcopyright} 2023 Nagy, Lozano Vigario, Sparrius, van Capel, van Ree, Tas, de Vries, Geijtenbeek, Sl{\"u}tter, de Jong and for the DC4Balance consortium.",

year = "2023",

doi = "10.3389/fimmu.2023.1137538",

language = "English",

volume = "14",

journal = "Frontiers in Immunology",

issn = "1664-3224",

publisher = "Frontiers Media SA",

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TY - JOUR

T1 - Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells

AU - DC4Balance consortium

AU - Nagy, Noémi Anna

AU - Lozano Vigario, Fernando

AU - Sparrius, Rinske

AU - van Capel, Toni M M

AU - van Ree, Ronald

AU - Tas, Sander W

AU - de Vries, I Jolanda M

AU - Geijtenbeek, Teunis B H

AU - Slütter, Bram

AU - de Jong, Esther C

N1 - Funding Information: This work was supported by Health Holland and “Samenwerkende Gezondheidsfonden” (SGF), with the LSH-TKI project the LSH-TKI project DC4Balance LSHM18056-SGF. The sponsors had no role in the study design, collection, analysis, interpretation of data, report writing, or decision to submit the manuscript for publication. Acknowledgments Publisher Copyright: Copyright © 2023 Nagy, Lozano Vigario, Sparrius, van Capel, van Ree, Tas, de Vries, Geijtenbeek, Slütter, de Jong and for the DC4Balance consortium.

PY - 2023

Y1 - 2023

N2 - INTRODUCTION: Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.METHODS: Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.RESULTS: Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.DISCUSSION: These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.

AB - INTRODUCTION: Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.METHODS: Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.RESULTS: Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.DISCUSSION: These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.

KW - T regulatory cells (Tregs)

KW - allergies

KW - autoimmunity

KW - dendritic cells

KW - immunotherapy

KW - liposome

KW - tolerance

KW - vitamin D3

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U2 - 10.3389/fimmu.2023.1137538

DO - 10.3389/fimmu.2023.1137538

M3 - Article

C2 - 37359530

SN - 1664-3224

VL - 14

JO - Frontiers in Immunology

JF - Frontiers in Immunology

M1 - 1137538

ER -

Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells

Abstract

Bibliographical note

Keywords

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