Laser microdissection of fungal septa as visualised by scanning electron microscopy

Kenneth G.A. van Driel; Teun Boekhout; Han A.B. Wösten; Arie J. Verkleij; Wally H. Müller

doi:10.1016/j.fgb.2006.10.006

Laser microdissection of fungal septa as visualised by scanning electron microscopy

Kenneth G.A. van Driel^*
, Teun Boekhout
, Han A.B. Wösten
, Arie J. Verkleij
, Wally H. Müller

^*Corresponding author for this work

Centraalbureau voor Schimmelcultures

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting. Subsequently, microdissected septa were visualised using a wheat germ agglutinin labelling of cell walls, septa and septal pore caps and scanning electron microscopy. The use of laser microdissection on fungal cells opens new ways to study subcellular fungal structures and the biochemical composition of hyphal cells.

Original language	English
Pages (from-to)	466-473
Number of pages	8
Journal	Fungal Genetics and Biology
Volume	44
Issue number	6
DOIs	https://doi.org/10.1016/j.fgb.2006.10.006
Publication status	Published - 1 Jun 2007

Funding

We express our thanks to Dr. Elly Hol (Netherlands Institute for Brain Research) for technical advice and using the PALM Microbeam system (Figures 3 and 5). We also like to thank DSM Food Specialties (Delft, The Netherlands) and SenterNovem (The Netherlands) for using their PALM Microbeam system (Figure 7). This work was financially supported by the Odo van Vloten foundation.

Keywords

Electron microscopy
Fungi
Laser microdissection
Laser pressure catapulting
PALM
Rhizoctonia solani
Septal pore cap
Septum
Wheat germ agglutinin

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10.1016/j.fgb.2006.10.006

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title = "Laser microdissection of fungal septa as visualised by scanning electron microscopy",

abstract = "Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting. Subsequently, microdissected septa were visualised using a wheat germ agglutinin labelling of cell walls, septa and septal pore caps and scanning electron microscopy. The use of laser microdissection on fungal cells opens new ways to study subcellular fungal structures and the biochemical composition of hyphal cells.",

keywords = "Electron microscopy, Fungi, Laser microdissection, Laser pressure catapulting, PALM, Rhizoctonia solani, Septal pore cap, Septum, Wheat germ agglutinin",

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AU - van Driel, Kenneth G.A.

AU - Boekhout, Teun

AU - Wösten, Han A.B.

AU - Verkleij, Arie J.

AU - Müller, Wally H.

PY - 2007/6/1

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N2 - Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting. Subsequently, microdissected septa were visualised using a wheat germ agglutinin labelling of cell walls, septa and septal pore caps and scanning electron microscopy. The use of laser microdissection on fungal cells opens new ways to study subcellular fungal structures and the biochemical composition of hyphal cells.

AB - Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting. Subsequently, microdissected septa were visualised using a wheat germ agglutinin labelling of cell walls, septa and septal pore caps and scanning electron microscopy. The use of laser microdissection on fungal cells opens new ways to study subcellular fungal structures and the biochemical composition of hyphal cells.

KW - Electron microscopy

KW - Fungi

KW - Laser microdissection

KW - Laser pressure catapulting

KW - PALM

KW - Rhizoctonia solani

KW - Septal pore cap

KW - Septum

KW - Wheat germ agglutinin

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SN - 1087-1845

VL - 44

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EP - 473

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

IS - 6

ER -

Laser microdissection of fungal septa as visualised by scanning electron microscopy

Abstract

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Keywords

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