Lactobacillus rhamnosus and Bifidobacterium breve suppress cigarette smoke induced CXCL-8 release by human macrophages

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Abstract

Introduction: Chronic obstructive pulmonary disease (COPD) is a major health problem and cigarette smoke (CS) is the main risk factor for the development of COPD1. Airway inflammation in COPD involves inflammatory mediators such as CXCL-8 and IL-1β which are generally considered to be important mediators for neutrophil recruitment2. Besides neutrophils macrophages play a pivotal role in the pathogenesis of COPD as well. In the macrophage, inflammasome is a multi-protein complex that is involved in the production of IL1β and other cytokines during the chronic phase of the diseases (Fig. 1). Interestingly, IL-1β can induce the release of CXCL-8 from bronchial cells3. Probiotics have been introduced in the treatment and prevention of gastrointestinal diseases4, common cold and allergies5. The mechanisms of action of probiotics are mainly unknown however their immuno-modulating effects have been described6-8. Objective and material and methods: The effects of Lactobacillus rhamnosus (BF02) and Bifidobacterium breve (BF06) on the cigarette smoke extract (CSE)-induced CXCL-8 and IL-1β release by human macrophages (THP-1) was investigated. Results: CSE and BF06 induced expression of IL-1β and release of CXCL-8 by THP-1 macrophages (Fig. 2). Surprisingly, preincubation of the THP-1 cellswith either BF02 or BF06 suppressed the cigarette smoke induced CXCL-8 release (Fig. 2). Pre-incubation of THP-1 with Z-VADFMK (an inflammasome inhibitor) suppressed the release of CXCL-8- induced by CSE and BF06 (Fig. 3A). BF06 and CSE induced NF-κB activity in THP-1 and preincubation of cells with BF02 and BF06 attenuated activity of NF-κB induced by CSE (Fig. 3B). BF02 and BF06 attenuated the CSE-induced expression of IL-1β as well (Fig. 4). Conclusion: In conclusion, the data presented here suggest that BF06 might target inflammasome activation in macrophages. Besides the current in vitro data may open a new line of studies to investigate the effectiveness of these bacteria as a therapeutic option in the treatment of COPD. (Figure presented) .
Original languageEnglish
Pages11-12
Number of pages2
DOIs
Publication statusPublished - 1 Sept 2011

Keywords

  • Bacteria
  • Cigarette Smoke
  • Emphysema
  • cigarette smoke
  • interleukin 1
  • inflammasome
  • probiotic agent
  • protein
  • cytokine
  • Bifidobacterium breve
  • macrophage
  • emphysema
  • human
  • Lactobacillus rhamnosus
  • bacterium
  • nutrition
  • neutrophil
  • health
  • common cold
  • in vitro study
  • pathogenesis
  • prevention
  • airway
  • inflammation
  • risk factor
  • chronic obstructive lung disease

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