Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

Maria Laura Tognoli, Julia Dancourt, Emeline Bonsergent, Roberta Palmulli, Olivier G. de Jong, Guillaume Van Niel, Eric Rubinstein, Pieter Vader, Gregory Lavieu

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.

Original languageEnglish
Article number532
Pages (from-to)1-9
Number of pages9
JournalCommunications Biology
Volume6
Issue number1
DOIs
Publication statusPublished - 17 May 2023

Bibliographical note

Publisher Copyright:
© 2023, The Author(s).

Funding

Work in G.L. lab is supported by INSERM, French National Research Agency (Excelldisc, BIOEV, EVfusion), and Chaire d’Excellence Idex Université Paris Cité. G.L thanks the Iveth facility and EVerZom for NTA access. The work of M.L.T. and P.V. was supported by an NWO OCENW-KLEIN grant and the European Research Council (ERC) Starting grant OBSERVE (# 851936) to P.V.

FundersFunder number
European Research Council851936
Agence Nationale de la Recherche
Institut national de la santé et de la recherche médicale
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

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