Laboratory evaluation of a rapid diagnostic test for dairy mastitis

David C Speksnijder*, Hendrika C Verduijn, Sharon van Haren, Tomas Ussing, Tine van Werven

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Rapid diagnostic tests that differentiate between Gram positive, Gram negative and the absence of aerobic bacteria in milk samples from dairy cows with clinical mastitis can support antimicrobial treatment decisions and contribute to a more prudent use of antimicrobials in the dairy industry. The objective of this study was to evaluate the test characteristics of the novel rapid BACT mastitis test in discriminating causes of clinical mastitis under laboratory conditions. Test outcomes of 155 milk samples from clinical mastitis cases were incubated for 14-16 h in the BACT test and compared to results of bacteriological culture. The accuracy for detection of bacterial growth and Gram positive growth was 91 and 89%, respectively. The BACT test could provide an accurate and relatively fast decision tool for farmers to aid in antimicrobial treatment decisions in cases of clinical mastitis.

Original languageEnglish
Pages (from-to)67-69
Number of pages3
JournalJournal of Dairy Research
Volume91
Issue number1
DOIs
Publication statusPublished - Feb 2024

Bibliographical note

Publisher Copyright:
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

Funding

The authors thank the three external veterinary laboratories that participated in performing this experiment: Dierenartsenpraktijk Dokkum, Slingeland Dierenartsen & Brabants Veterinair Laboratorium. FluimediX provided the BACT test devices and financial funding for the laboratory experiments. Fluimedix did not have any influence in the design of the experiment, the interpretation of laboratory results, data analysis or publication decisions.

FundersFunder number
BACT test devices

    Keywords

    • antimicrobial stewardship
    • bovine mastitis
    • on-farm testing
    • rapid diagnostics

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