Label-Free Time-Resolved Monitoring of Photolipid Bilayer Isomerization by Plasmonic Sensing

Jinhua Zhang; Francis Schuknecht; Ludwig Habermann; Alexander Pattis; Jonathan Heine; Stefanie D. Pritzl; Dirk Trauner; Theobald Lohmüller

doi:10.1002/adom.202302266

Label-Free Time-Resolved Monitoring of Photolipid Bilayer Isomerization by Plasmonic Sensing

Jinhua Zhang, Francis Schuknecht, Ludwig Habermann, Alexander Pattis, Jonathan Heine, Stefanie D. Pritzl, Dirk Trauner, Theobald Lohmüller^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The photo-isomerization of the photolipid azo-PC, a derivative of phosphatidylcholine containing an azobenzene group in its sn2 acyl chain, enables optical control over key properties of supported lipid bilayers (SLBs), such as membrane fluidity and bilayer thickness. However, azobenzenes are well-known for their interaction with various dyes through photo-modulation and -sensitization pathways, presenting a challenge in bilayer characterization by fluorescence microscopy. Label-free tools capable of monitoring the switching process of photolipid SLBs at the nanoscale are therefore highly desired. In this study, the use of dark field scattering spectroscopy on gold nanorods as a highly sensitive approach is demonstrated for analyzing the reversible photo-isomerization dynamics of photolipid SLBs in real time at the single particle level.

Original language	English
Article number	2302266
Journal	Advanced Optical Materials
Volume	12
Issue number	10
DOIs	https://doi.org/10.1002/adom.202302266
Publication status	Published - 4 Apr 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors. Advanced Optical Materials published by Wiley-VCH GmbH.

Funding

This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Center SFB1032 (Project no. 201269156, Project A8). The authors thank local research clusters and centers such as the Center of Nanoscience (CeNS) for providing communicative networking structures. S.D.P. is supported by the Alexander-von-Humboldt foundation through a Feodor-Lynen fellowship.\u00A0J.Z. is supported by the China Scholarship Council. Open access funding enabled and organized by Projekt DEAL. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Center SFB1032 (Project no. 201269156, Project A8). The authors thank local research clusters and centers such as the Center of Nanoscience (CeNS) for providing communicative networking structures. S.D.P. is supported by the Alexander\u2010von\u2010Humboldt foundation through a Feodor\u2010Lynen fellowship. J.Z. is supported by the China Scholarship Council.

Funders	Funder number
Centre for Nano and Soft Matter Sciences
Deutsche Forschungsgemeinschaft
China Scholarship Council
Alexander von Humboldt-Stiftung
Collaborative Research Center	SFB1032, 201269156

Keywords

azobenzene
photolipids
plasmonic sensing
supported bilayer membranes

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Advanced Optical Materials - 2024 - Zhang - Label‐Free Time‐Resolved Monitoring of Photolipid Bilayer Isomerization byFinal published version, 1.59 MBLicence: CC BY-NC-ND

Cite this

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abstract = "The photo-isomerization of the photolipid azo-PC, a derivative of phosphatidylcholine containing an azobenzene group in its sn2 acyl chain, enables optical control over key properties of supported lipid bilayers (SLBs), such as membrane fluidity and bilayer thickness. However, azobenzenes are well-known for their interaction with various dyes through photo-modulation and -sensitization pathways, presenting a challenge in bilayer characterization by fluorescence microscopy. Label-free tools capable of monitoring the switching process of photolipid SLBs at the nanoscale are therefore highly desired. In this study, the use of dark field scattering spectroscopy on gold nanorods as a highly sensitive approach is demonstrated for analyzing the reversible photo-isomerization dynamics of photolipid SLBs in real time at the single particle level.",

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AU - Habermann, Ludwig

AU - Pattis, Alexander

AU - Heine, Jonathan

AU - Pritzl, Stefanie D.

AU - Trauner, Dirk

AU - Lohmüller, Theobald

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Label-Free Time-Resolved Monitoring of Photolipid Bilayer Isomerization by Plasmonic Sensing

Abstract

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Keywords

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