Label-free optical imaging of mitochondria in live cells

David Lasne; Gerhard A. Blab; Francesca De Giorgi; François Ichas; Brahim Lounis; Laurent Cognet

doi:10.1364/OE.15.014184

Label-free optical imaging of mitochondria in live cells

David Lasne, Gerhard A. Blab, Francesca De Giorgi, François Ichas, Brahim Lounis, Laurent Cognet^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.

Original language	English
Pages (from-to)	14184-14193
Number of pages	10
Journal	Optics Express
Volume	15
Issue number	21
DOIs	https://doi.org/10.1364/OE.15.014184
Publication status	Published - 17 Oct 2007

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abstract = "The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.",

author = "David Lasne and Blab, {Gerhard A.} and {De Giorgi}, Francesca and Fran{\c c}ois Ichas and Brahim Lounis and Laurent Cognet",

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AU - De Giorgi, Francesca

AU - Ichas, François

AU - Lounis, Brahim

AU - Cognet, Laurent

PY - 2007/10/17

Y1 - 2007/10/17

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AB - The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.

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Label-free optical imaging of mitochondria in live cells

Abstract

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