Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS)

Xiuru Li; Sharon J H Martin; Zoeisha S Chinoy; Lin Liu; Brandon Rittgers; Richard A Dluhy; Geert-Jan Boons

doi:10.1002/chem.201602706

Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS)

Xiuru Li, Sharon J H Martin, Zoeisha S Chinoy, Lin Liu, Brandon Rittgers, Richard A Dluhy, Geert-Jan Boons

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A glyco-array platform has been developed, in which glycans are attached to plasmonic nanoparticles through strain-promoted azide-alkyne cycloaddition. Glycan-protein binding events can then be detected in a label-free manner employing surface-enhanced Raman spectroscopy (SERS). As proof of concept, we have analyzed the binding of Gal1, Gal3, and influenza hemagglutinins (HAs) to various glycans and demonstrated that binding partners can be identified with high confidence. The attraction of SERS for optical sensing is that it can provide unique spectral signatures for glycan-protein complexes, confirm identity through statistical validation, and minimizes false positive results common to indirect methods. Furthermore, SERS is very sensitive and has multiplexing capabilities thereby allowing the simultaneous detection of multiple analytes.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Chemistry-A European Journal
Volume	22
DOIs	https://doi.org/10.1002/chem.201602706
Publication status	Published - 15 Jun 2016

Access to Document

10.1002/chem.201602706

Li_et_alFinal published version, 1.7 MBLicence: Taverne

Cite this

@article{9b6cad11508d430f816e1770bbb6529c,

title = "Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS)",

abstract = "A glyco-array platform has been developed, in which glycans are attached to plasmonic nanoparticles through strain-promoted azide-alkyne cycloaddition. Glycan-protein binding events can then be detected in a label-free manner employing surface-enhanced Raman spectroscopy (SERS). As proof of concept, we have analyzed the binding of Gal1, Gal3, and influenza hemagglutinins (HAs) to various glycans and demonstrated that binding partners can be identified with high confidence. The attraction of SERS for optical sensing is that it can provide unique spectral signatures for glycan-protein complexes, confirm identity through statistical validation, and minimizes false positive results common to indirect methods. Furthermore, SERS is very sensitive and has multiplexing capabilities thereby allowing the simultaneous detection of multiple analytes.",

author = "Xiuru Li and Martin, {Sharon J H} and Chinoy, {Zoeisha S} and Lin Liu and Brandon Rittgers and Dluhy, {Richard A} and Geert-Jan Boons",

note = "{\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2016",

month = jun,

day = "15",

doi = "10.1002/chem.201602706",

language = "English",

volume = "22",

pages = "1--7",

journal = "Chemistry-A European Journal",

issn = "0947-6539",

publisher = "Wiley-VCH Verlag",

}

TY - JOUR

T1 - Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS)

AU - Li, Xiuru

AU - Martin, Sharon J H

AU - Chinoy, Zoeisha S

AU - Liu, Lin

AU - Rittgers, Brandon

AU - Dluhy, Richard A

AU - Boons, Geert-Jan

PY - 2016/6/15

Y1 - 2016/6/15

N2 - A glyco-array platform has been developed, in which glycans are attached to plasmonic nanoparticles through strain-promoted azide-alkyne cycloaddition. Glycan-protein binding events can then be detected in a label-free manner employing surface-enhanced Raman spectroscopy (SERS). As proof of concept, we have analyzed the binding of Gal1, Gal3, and influenza hemagglutinins (HAs) to various glycans and demonstrated that binding partners can be identified with high confidence. The attraction of SERS for optical sensing is that it can provide unique spectral signatures for glycan-protein complexes, confirm identity through statistical validation, and minimizes false positive results common to indirect methods. Furthermore, SERS is very sensitive and has multiplexing capabilities thereby allowing the simultaneous detection of multiple analytes.

AB - A glyco-array platform has been developed, in which glycans are attached to plasmonic nanoparticles through strain-promoted azide-alkyne cycloaddition. Glycan-protein binding events can then be detected in a label-free manner employing surface-enhanced Raman spectroscopy (SERS). As proof of concept, we have analyzed the binding of Gal1, Gal3, and influenza hemagglutinins (HAs) to various glycans and demonstrated that binding partners can be identified with high confidence. The attraction of SERS for optical sensing is that it can provide unique spectral signatures for glycan-protein complexes, confirm identity through statistical validation, and minimizes false positive results common to indirect methods. Furthermore, SERS is very sensitive and has multiplexing capabilities thereby allowing the simultaneous detection of multiple analytes.

U2 - 10.1002/chem.201602706

DO - 10.1002/chem.201602706

M3 - Article

C2 - 27304194

SN - 0947-6539

VL - 22

SP - 1

EP - 7

JO - Chemistry-A European Journal

JF - Chemistry-A European Journal

ER -

Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS)

Abstract

Access to Document

Fingerprint

Cite this