L-arginine is not the limiting factor for nitric oxide synthesis by human alveolar macrophages in vitro

R.B.R. Muijsers; N.H.T. Ten Hacken; I. Van Ark; G. Folkerts; F.P. Nijkamp; D.S. Postma

doi:10.1183/09031936.01.00101301

L-arginine is not the limiting factor for nitric oxide synthesis by human alveolar macrophages in vitro

R.B.R. Muijsers, N.H.T. Ten Hacken, I. Van Ark, G. Folkerts, F.P. Nijkamp, D.S. Postma

Pharmacology

External unknown

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Unlike murine mononuclear phagocytes, human macrophages do not release high amounts of nitric oxide (NO) in vitro despite the presence of nitric oxide synthase (NOS). To determine whether this limited NO synthesis in vitro is due to limited availability of the NOS substrate L-arginine, and putative NOS inhibiting factors present in foetal serum preparations, both alveolar macrophages (AM) and monocyte derived macrophages (MDM) were incubated in various circumstances. Nitrite production measured using stimulated AM was typically

Original language	English
Pages (from-to)	667-671
Number of pages	5
Journal	European Respiratory Journal
Volume	18
Issue number	4
DOIs	https://doi.org/10.1183/09031936.01.00101301
Publication status	Published - 28 Mar 2001

Keywords

Alveolar macrophage
Human
Monocyte
Nitric oxide
aminoguanidine
arginase
arginine
bovine serum albumin
enzyme inhibitor
n(g) hydroxyarginine
nitric oxide
nitric oxide synthase
nitric oxide synthase inhibitor
nitrite
animal cell
article
biosynthesis
cellular secretion
cell stimulation
controlled study
enzyme inhibition
enzyme substrate
fetal calf serum
human
human cell
in vitro study
incubation time
lung alveolus macrophage
macrophage
monocyte
nonhuman
priority journal

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10.1183/09031936.01.00101301

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title = "L-arginine is not the limiting factor for nitric oxide synthesis by human alveolar macrophages in vitro",

abstract = "Unlike murine mononuclear phagocytes, human macrophages do not release high amounts of nitric oxide (NO) in vitro despite the presence of nitric oxide synthase (NOS). To determine whether this limited NO synthesis in vitro is due to limited availability of the NOS substrate L-arginine, and putative NOS inhibiting factors present in foetal serum preparations, both alveolar macrophages (AM) and monocyte derived macrophages (MDM) were incubated in various circumstances. Nitrite production measured using stimulated AM was typically",

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author = "R.B.R. Muijsers and {Ten Hacken}, N.H.T. and {Van Ark}, I. and G. Folkerts and F.P. Nijkamp and D.S. Postma",

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doi = "10.1183/09031936.01.00101301",

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AU - Muijsers, R.B.R.

AU - Ten Hacken, N.H.T.

AU - Van Ark, I.

AU - Folkerts, G.

AU - Nijkamp, F.P.

AU - Postma, D.S.

PY - 2001/3/28

Y1 - 2001/3/28

N2 - Unlike murine mononuclear phagocytes, human macrophages do not release high amounts of nitric oxide (NO) in vitro despite the presence of nitric oxide synthase (NOS). To determine whether this limited NO synthesis in vitro is due to limited availability of the NOS substrate L-arginine, and putative NOS inhibiting factors present in foetal serum preparations, both alveolar macrophages (AM) and monocyte derived macrophages (MDM) were incubated in various circumstances. Nitrite production measured using stimulated AM was typically

AB - Unlike murine mononuclear phagocytes, human macrophages do not release high amounts of nitric oxide (NO) in vitro despite the presence of nitric oxide synthase (NOS). To determine whether this limited NO synthesis in vitro is due to limited availability of the NOS substrate L-arginine, and putative NOS inhibiting factors present in foetal serum preparations, both alveolar macrophages (AM) and monocyte derived macrophages (MDM) were incubated in various circumstances. Nitrite production measured using stimulated AM was typically

KW - Alveolar macrophage

KW - Human

KW - Monocyte

KW - Nitric oxide

KW - aminoguanidine

KW - arginase

KW - arginine

KW - bovine serum albumin

KW - enzyme inhibitor

KW - n(g) hydroxyarginine

KW - nitric oxide

KW - nitric oxide synthase

KW - nitric oxide synthase inhibitor

KW - nitrite

KW - animal cell

KW - article

KW - biosynthesis

KW - cellular secretion

KW - cell stimulation

KW - controlled study

KW - enzyme inhibition

KW - enzyme substrate

KW - fetal calf serum

KW - human

KW - human cell

KW - in vitro study

KW - incubation time

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EP - 671

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - 4

ER -

L-arginine is not the limiting factor for nitric oxide synthesis by human alveolar macrophages in vitro

Abstract

Keywords

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