Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis

A. Klein; C. Carnoy; G. Lamblin; P. Roussel; J.A. Van Kuik; J.F.G. Vliegenthart

doi:10.1111/j.1432-1033.1993.tb17575.x

Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis

A. Klein, C. Carnoy, G. Lamblin, P. Roussel, J.A. Van Kuik, J.F.G. Vliegenthart

Unite des Proteines

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis.

Original language	English
Pages (from-to)	491-500
Number of pages	10
Journal	European Journal of Biochemistry
Volume	211
Issue number	3
DOIs	https://doi.org/10.1111/j.1432-1033.1993.tb17575.x
Publication status	Published - 13 Jul 1993

Keywords

glycoprotein
article
bronchiectasis
case report
chemical structure
high performance liquid chromatography
human
Kartagener syndrome
mucus
priority journal
proton nuclear magnetic resonance
sputum

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10.1111/j.1432-1033.1993.tb17575.x

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title = "Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis",

abstract = "The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis.",

keywords = "glycoprotein, article, bronchiectasis, case report, chemical structure, high performance liquid chromatography, human, Kartagener syndrome, mucus, priority journal, proton nuclear magnetic resonance, sputum",

author = "A. Klein and C. Carnoy and G. Lamblin and P. Roussel and {Van Kuik}, J.A. and J.F.G. Vliegenthart",

year = "1993",

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doi = "10.1111/j.1432-1033.1993.tb17575.x",

language = "English",

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pages = "491--500",

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Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis. / Klein, A.; Carnoy, C.; Lamblin, G. et al.
In: European Journal of Biochemistry, Vol. 211, No. 3, 13.07.1993, p. 491-500.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis

AU - Klein, A.

AU - Carnoy, C.

AU - Lamblin, G.

AU - Roussel, P.

AU - Van Kuik, J.A.

AU - Vliegenthart, J.F.G.

PY - 1993/7/13

Y1 - 1993/7/13

N2 - The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis.

AB - The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis.

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KW - case report

KW - chemical structure

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KW - mucus

KW - priority journal

KW - proton nuclear magnetic resonance

KW - sputum

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DO - 10.1111/j.1432-1033.1993.tb17575.x

M3 - Article

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JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

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ER -

Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis

Abstract

Keywords

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