Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation

Anton J. M.  Peeters; Marcel Proveniers; Angela van Hoek; Max  Schreuder; Will  Gerards; Gerard W. M.  Barendse; George J.  Wullems

doi:10.1007/BF00199687

Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation

Anton J. M. Peeters, Marcel Proveniers, Angela van Hoek, Max Schreuder, Will Gerards, Gerard W. M. Barendse^*, George J. Wullems

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The development of vegetative and generative buds on thin-layer expiants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.

Original language	English
Pages (from-to)	271-281
Journal	Planta
Volume	195
DOIs	https://doi.org/10.1007/BF00199687
Publication status	Published - Dec 1994
Externally published	Yes

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@article{218de5b67c6d4ed4ad7cc15bdd6c005a,

title = "Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation",

abstract = "The development of vegetative and generative buds on thin-layer expiants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.",

author = "Peeters, {Anton J. M.} and Marcel Proveniers and {van Hoek}, Angela and Max Schreuder and Will Gerards and Barendse, {Gerard W. M.} and Wullems, {George J.}",

year = "1994",

month = dec,

doi = "10.1007/BF00199687",

language = "English",

volume = "195",

pages = "271--281",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer Science and Business Media Deutschland GmbH",

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TY - JOUR

T1 - Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation

AU - Peeters, Anton J. M.

AU - Proveniers, Marcel

AU - van Hoek, Angela

AU - Schreuder, Max

AU - Gerards, Will

AU - Barendse, Gerard W. M.

AU - Wullems , George J.

PY - 1994/12

Y1 - 1994/12

N2 - The development of vegetative and generative buds on thin-layer expiants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.

AB - The development of vegetative and generative buds on thin-layer expiants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.

U2 - 10.1007/BF00199687

DO - 10.1007/BF00199687

M3 - Article

SN - 0032-0935

VL - 195

SP - 271

EP - 281

JO - Planta

JF - Planta

ER -

Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation

Abstract

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