Involvement of Neisseria meningitidis Lipoprotein GNA2091 in the Assembly of a Subset of Outer Membrane Proteins

Martine P. Bos*, Jan Grijpstra, Hendrika Tommassen - van Boxtel, J Tommassen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

GNA2091 of Neisseria meningitidis is a lipoprotein of unknown function that is included in the novel 4CMenB vaccine. Here, we investigated the biological function and the subcellular localization of the protein. We demonstrate that GNA2091 functions in the assembly of outer membrane proteins (OMPs) because its absence resulted in the accumulation of misassembled OMPs. Cell fractionation and protease accessibility experiments showed that the protein is localized at the periplasmic side of the outer membrane. Pulldown experiments revealed that it is not stably associated with the -barrel assembly machinery, the previously identified complex for OMP assembly. Thus, GNA2091 constitutes a novel outer membrane-based lipoprotein required for OMP assembly. Furthermore, its location at the inner side of the outer membrane indicates that protective immunity elicited by this antigen cannot be due to bactericidal or opsonic activity of antibodies.

Original languageEnglish
Pages (from-to)15602-15610
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number22
DOIs
Publication statusPublished - 30 May 2014

Keywords

  • Bacteria
  • Membrane Biogenesis
  • Membrane Protein
  • Microbiology
  • Vaccine
  • BAM Complex
  • Neisseria meningitidis
  • YraP
  • ESCHERICHIA-COLI
  • BETA-BARREL
  • SEROGROUP-B
  • LIPOPOLYSACCHARIDE TRANSPORT
  • BAM COMPLEX
  • IDENTIFICATION
  • BIOGENESIS
  • VACCINE
  • CHAPERONE
  • BACTERIA

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