Interference of Salmonella enteritidis and Lactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like Caco-2 cells

S Fajdiga, J F J G Koninkx, P C J Tooten, R Marinsek-Logar

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (Salmonella enteritidis causing human gastroenteritis)-intestinal cell interactions. The interference of bacteria with the transepithelial electrical resistance (TEER) of filter-grown Caco-2 cells and the production of IL-8 after exposure of the cells to S. enteritidis 857 and/or Lactobacillus strains (L. gasseri LF221 and L. rhamnosus BGT10) was evaluated. The strain 857 decreased TEER of filter-grown Caco-2 cells; in contrast, lactobacilli had a little or no effect. The effect of S. enteritidis on the TEER decreased if Caco-2 cells were pre-incubated with lactobacilli. This strain induced high levels of IL-8 (which can lead to cell damage). Compared to the IL-8 synthesis after exposure of Caco-2 cells to S. enteritidis 857, simultaneous exposure of Caco-2 cells to S. enteritidis and lactobacilli inhibited the IL-8 synthesis after short recovery periods.

    Original languageEnglish
    Pages (from-to)268-272
    Number of pages5
    JournalFolia Microbiologica
    Volume51
    Issue number4
    Publication statusPublished - 2006

    Keywords

    • Caco-2 Cells
    • Electric Impedance
    • Humans
    • Immunity, Mucosal
    • Interleukin-8
    • Intestinal Mucosa
    • Lactobacillus
    • Salmonella Infections
    • Salmonella enteritidis

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