Abstract
Caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (Salmonella enteritidis causing human gastroenteritis)-intestinal cell interactions. The interference of bacteria with the transepithelial electrical resistance (TEER) of filter-grown Caco-2 cells and the production of IL-8 after exposure of the cells to S. enteritidis 857 and/or Lactobacillus strains (L. gasseri LF221 and L. rhamnosus BGT10) was evaluated. The strain 857 decreased TEER of filter-grown Caco-2 cells; in contrast, lactobacilli had a little or no effect. The effect of S. enteritidis on the TEER decreased if Caco-2 cells were pre-incubated with lactobacilli. This strain induced high levels of IL-8 (which can lead to cell damage). Compared to the IL-8 synthesis after exposure of Caco-2 cells to S. enteritidis 857, simultaneous exposure of Caco-2 cells to S. enteritidis and lactobacilli inhibited the IL-8 synthesis after short recovery periods.
Original language | English |
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Pages (from-to) | 268-272 |
Number of pages | 5 |
Journal | Folia Microbiologica |
Volume | 51 |
Issue number | 4 |
Publication status | Published - 2006 |
Keywords
- Caco-2 Cells
- Electric Impedance
- Humans
- Immunity, Mucosal
- Interleukin-8
- Intestinal Mucosa
- Lactobacillus
- Salmonella Infections
- Salmonella enteritidis