Interaction of the C-terminal domains of sendai virus N and P proteins: Comparison of polymerase-nucleocapsid interactions within the paramyxovirus family

Klaartje Houben, Dominique Marion, Nicolas Tarbouriech, Rob W. H. Ruigrok, Laurence Blanchard

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Interaction of the C-terminal domains of Sendai virus (SeV) P and N proteins is crucial for RNA synthesis by correctly positioning the polymerase complex (L+P) onto the nucleocapsid (N/RNA). To better understand this mechanism within the paramyxovirus family, we have studied the complex formed by the SeV C-terminal domains of P (PX) and N (NTAIL) proteins by solution nuclear magnetic resonance spectroscopy. We have characterized SeV NTAIL, which belongs to the class of intrinsically disordered proteins, and precisely defined the binding regions within this latter domain and within PX. SeV NTAILbinds with residues 472 to 493, which have a helical propensity (residues 477 to 491) to the surface created by helices α2 and α3 of PX with a 1:1 stoichiometry, as was also found for measles virus (MV). The binding interface is dominated by charged residues, and the dissociation constant was determined to be 57 ± 18 μM under conditions of the experiment (i.e., in 0.5 M NaCl). We have also shown that the extreme C terminus of SeV NTAILdoes not interact with PX, which is in contrast to MV, where a second binding site was identified. In addition, the interaction surfaces of the MV proteins are hydrophobic and a stronger binding constant was found. This gives a good illustration of how selection pressure allowed the C-terminal domains of N and P proteins to evolve concomitantly within this family of viruses in order to lead to protein complexes having the same three-dimensional fold, and thus the same function, but with completely different binding interfaces. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)6807-6816
Number of pages10
JournalJournal of Virology
Volume81
Issue number13
DOIs
Publication statusPublished - 1 Jul 2007
Externally publishedYes

Keywords

  • phosphoprotein
  • RNA polymerase
  • sodium chloride
  • unclassified drug
  • virus protein
  • virus protein N
  • virus protein P
  • virus RNA
  • amino acid sequence
  • article
  • carboxy terminal sequence
  • Measles virus
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • Paramyxoviridae
  • priority journal
  • protein binding
  • protein domain
  • protein function
  • protein protein interaction
  • RNA synthesis
  • Sendai virus
  • virus nucleocapsid

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