Interaction of antithrombin III with preadsorbed albumin-heparin conjugates

W. E. Hennink; C. D. Ebert; S. W. Kim; W. Breemhaar; A. Bantjes; J. Feijen

doi:10.1016/0142-9612(84)90070-X

Interaction of antithrombin III with preadsorbed albumin-heparin conjugates

W. E. Hennink^*
, C. D. Ebert
, S. W. Kim
, W. Breemhaar
, A. Bantjes
, J. Feijen

^*Corresponding author for this work

University of Utah

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The adsorption of antithrombin III (AT III) onto polystyrene surfaces preadsorbed with albumin or albuminheparin conjugates was studied using a two step enzyme immuno assay. When AT III-buffer solutions were used, the highest adsorption values were measured on high affinity albumin-heparin conjugate pretreated surfaces. Less AT III adsorption was found on nonfractionated albumin-heparin conjugate preadsorbed surfaces. AT III adsorption could also be detected on low affinity conjugate and albumin coated surfaces. When AT III was adsorbed from plasma or plasma dilutions with buffer, only AT III on surfaces preadsorbed with high affinity or nonfractionated albumin-heparin conjugate was found. These results demonstrate that the heparin moiety of the conjugate is directed to the solution phase whereas the albumin moiety contacts the polystyrene surfaca.

Original language	English
Pages (from-to)	264-268
Number of pages	5
Journal	Biomaterials
Volume	5
Issue number	5
DOIs	https://doi.org/10.1016/0142-9612(84)90070-X
Publication status	Published - Sept 1984

Bibliographical note

Funding Information:
The authors thank the American Red Cross for their gift of purified AT III. This research was supported by Grant HL-17623-09 of the National Institutes of Health. One of the authors (W.B.) thanks Organon Technika for financial support.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Funding

The authors thank the American Red Cross for their gift of purified AT III. This research was supported by Grant HL-17623-09 of the National Institutes of Health. One of the authors (W.B.) thanks Organon Technika for financial support.

Keywords

albumin-heparin conjugates
antithrombin III
heparin
Polymers
protein adsorption

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10.1016/0142-9612(84)90070-X

Interaction of antithrombin III with preadsorbed albumin-heparin conjugatesFinal published version, 593 KBLicence: Taverne

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title = "Interaction of antithrombin III with preadsorbed albumin-heparin conjugates",

abstract = "The adsorption of antithrombin III (AT III) onto polystyrene surfaces preadsorbed with albumin or albuminheparin conjugates was studied using a two step enzyme immuno assay. When AT III-buffer solutions were used, the highest adsorption values were measured on high affinity albumin-heparin conjugate pretreated surfaces. Less AT III adsorption was found on nonfractionated albumin-heparin conjugate preadsorbed surfaces. AT III adsorption could also be detected on low affinity conjugate and albumin coated surfaces. When AT III was adsorbed from plasma or plasma dilutions with buffer, only AT III on surfaces preadsorbed with high affinity or nonfractionated albumin-heparin conjugate was found. These results demonstrate that the heparin moiety of the conjugate is directed to the solution phase whereas the albumin moiety contacts the polystyrene surfaca.",

keywords = "albumin-heparin conjugates, antithrombin III, heparin, Polymers, protein adsorption",

author = "Hennink, \{W. E.\} and Ebert, \{C. D.\} and Kim, \{S. W.\} and W. Breemhaar and A. Bantjes and J. Feijen",

note = "Funding Information: The authors thank the American Red Cross for their gift of purified AT III. This research was supported by Grant HL-17623-09 of the National Institutes of Health. One of the authors (W.B.) thanks Organon Technika for financial support. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1984",

month = sep,

doi = "10.1016/0142-9612(84)90070-X",

language = "English",

volume = "5",

pages = "264--268",

journal = "Biomaterials",

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T1 - Interaction of antithrombin III with preadsorbed albumin-heparin conjugates

AU - Hennink, W. E.

AU - Ebert, C. D.

AU - Kim, S. W.

AU - Breemhaar, W.

AU - Bantjes, A.

AU - Feijen, J.

N1 - Funding Information: The authors thank the American Red Cross for their gift of purified AT III. This research was supported by Grant HL-17623-09 of the National Institutes of Health. One of the authors (W.B.) thanks Organon Technika for financial support. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1984/9

Y1 - 1984/9

N2 - The adsorption of antithrombin III (AT III) onto polystyrene surfaces preadsorbed with albumin or albuminheparin conjugates was studied using a two step enzyme immuno assay. When AT III-buffer solutions were used, the highest adsorption values were measured on high affinity albumin-heparin conjugate pretreated surfaces. Less AT III adsorption was found on nonfractionated albumin-heparin conjugate preadsorbed surfaces. AT III adsorption could also be detected on low affinity conjugate and albumin coated surfaces. When AT III was adsorbed from plasma or plasma dilutions with buffer, only AT III on surfaces preadsorbed with high affinity or nonfractionated albumin-heparin conjugate was found. These results demonstrate that the heparin moiety of the conjugate is directed to the solution phase whereas the albumin moiety contacts the polystyrene surfaca.

AB - The adsorption of antithrombin III (AT III) onto polystyrene surfaces preadsorbed with albumin or albuminheparin conjugates was studied using a two step enzyme immuno assay. When AT III-buffer solutions were used, the highest adsorption values were measured on high affinity albumin-heparin conjugate pretreated surfaces. Less AT III adsorption was found on nonfractionated albumin-heparin conjugate preadsorbed surfaces. AT III adsorption could also be detected on low affinity conjugate and albumin coated surfaces. When AT III was adsorbed from plasma or plasma dilutions with buffer, only AT III on surfaces preadsorbed with high affinity or nonfractionated albumin-heparin conjugate was found. These results demonstrate that the heparin moiety of the conjugate is directed to the solution phase whereas the albumin moiety contacts the polystyrene surfaca.

KW - albumin-heparin conjugates

KW - antithrombin III

KW - heparin

KW - Polymers

KW - protein adsorption

UR - https://www.scopus.com/pages/publications/0021223020

U2 - 10.1016/0142-9612(84)90070-X

DO - 10.1016/0142-9612(84)90070-X

M3 - Article

C2 - 6487709

AN - SCOPUS:0021223020

SN - 0142-9612

VL - 5

SP - 264

EP - 268

JO - Biomaterials

JF - Biomaterials

IS - 5

ER -

Interaction of antithrombin III with preadsorbed albumin-heparin conjugates

Abstract

Bibliographical note

Funding

Keywords

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