Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina

Catalina Landeta; David Medina-Ortiz; Natalia Escobar; Iván Valdez; María Paz González-Troncoso; Diego Álvares-Saravia; Jacqueline Aldridge; Carlos Gómez; María Elena Lienqueo

doi:10.1007/s00203-024-04087-0

Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina

Catalina Landeta, David Medina-Ortiz, Natalia Escobar, Iván Valdez, María Paz González-Troncoso, Diego Álvares-Saravia, Jacqueline Aldridge, Carlos Gómez, María Elena Lienqueo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Hydrophobins (HFBs) and cerato-platanins (CPs) are surface-active extracellular proteins produced by filamentous fungi. This study identified two HFB genes (pshyd1 and pshyd2) and one CP gene (pscp) in the marine fungus Paradendryphiella salina. The proteins PsCP, PsHYD2, and PsHYD1 had molecular weights of 12.70, 6.62, and 5.98 kDa, respectively, with isoelectric points below 7. PsHYD1 and PsHYD2 showed hydrophobicity (GRAVY score 0.462), while PsCP was hydrophilic (GRAVY score − 0.202). Stability indices indicated in-solution stability. Mass spectrometry identified 2,922 proteins, including CP but not HFB proteins. qPCR revealed differential gene expression influenced by developmental stage and substrate, with pshyd1 consistently expressed. These findings suggest P. salina’s adaptation to marine ecosystems with fewer hydrophobin genes than other fungi but capable of producing surface-active proteins from seaweed carbohydrates. These proteins have potential applications in medical biocoatings, food industry foam stabilizers, and environmental bioremediation.

Original language	English
Article number	385
Journal	Archives of Microbiology
Volume	206
Issue number	9
DOIs	https://doi.org/10.1007/s00203-024-04087-0
Publication status	Published - Sept 2024

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.

Funding

This research was supported by the National Research and Development Agency of Chile of the Government of Chile \u2013 ANID \u2013 (Grant number 21161794) and the Centre for Biotechnology and Bioengineering \u2013 CeBiB \u2013 (Project FB0001). We thank Professor Han A.B. W\u00F6sten of Department of Biology, Utrecht University, for his invaluable contributions.

Funders	Funder number
National Research and Development Agency of Chile of the Government of Chile
Agencia Nacional de Investigación y Desarrollo	21161794
Centre for Biotechnology and Bioengineering	FB0001

Keywords

Cerato-platanin
Hydrophobin
Marine fungi
Paradendryphiella salina

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00203-024-04087-0

s00203-024-04087-0Final published version, 2.74 MBLicence: Taverne

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Landeta, C., Medina-Ortiz, D., Escobar, N., Valdez, I., González-Troncoso, M. P., Álvares-Saravia, D., Aldridge, J., Gómez, C., & Lienqueo, M. E. (2024). Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina. Archives of Microbiology, 206(9), Article 385. https://doi.org/10.1007/s00203-024-04087-0

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abstract = "Hydrophobins (HFBs) and cerato-platanins (CPs) are surface-active extracellular proteins produced by filamentous fungi. This study identified two HFB genes (pshyd1 and pshyd2) and one CP gene (pscp) in the marine fungus Paradendryphiella salina. The proteins PsCP, PsHYD2, and PsHYD1 had molecular weights of 12.70, 6.62, and 5.98 kDa, respectively, with isoelectric points below 7. PsHYD1 and PsHYD2 showed hydrophobicity (GRAVY score 0.462), while PsCP was hydrophilic (GRAVY score − 0.202). Stability indices indicated in-solution stability. Mass spectrometry identified 2,922 proteins, including CP but not HFB proteins. qPCR revealed differential gene expression influenced by developmental stage and substrate, with pshyd1 consistently expressed. These findings suggest P. salina{\textquoteright}s adaptation to marine ecosystems with fewer hydrophobin genes than other fungi but capable of producing surface-active proteins from seaweed carbohydrates. These proteins have potential applications in medical biocoatings, food industry foam stabilizers, and environmental bioremediation.",

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AU - Álvares-Saravia, Diego

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AU - Gómez, Carlos

AU - Lienqueo, María Elena

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Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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