Insoluble hydrophobin complexes in the walls of Schizophyllum commune and other filamentous fungi

O.M.H. De Vries, M.P. Fekkes, H.A.B. Wosten, J.G.H. Wessels

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Two closely related cysteine-rich hydrophobic proteins, Sc3p and Sc4p, of the basidiomycete Schizophyllum commune are developmentally regulated and associated with the walls of aerial hyphae and fruit-body hyphae. They are present in the walls as hot-SDS insoluble complexes which can be extracted with formic acid. The hydrophobins can then be dissociated by oxidation with performic acid. However, extraction of the walls with trifluoroacetic acid results in both solubilization and dissociation of the hydrophobin complexes into monomers. This suggests that non-covalent interactions are responsible for formation of these insoluble complexes. Carboxymethylation with iodoacetic acid only occurred after reduction with DTT indicating all cysteines in the monomeric hydrophobins involved in intramolecular disulfide bridges. Abundant proteins with similar properties were found in walls from all other filamentous fungi tested, including the basidiomycetes Pleurotus ostreatus, Coprinus cinereus, Agaricus bisporus, and Pharnerochaete chrysosporium, the ascomycetes Aspergillus nidulans, Neurospora crassa, and Penicillium chrysogenum, and the zygomycete Mucor mucedo.
Original languageEnglish
Pages (from-to)330-335
Number of pages6
JournalArchives of Microbiology
Volume159
Issue number4
Publication statusPublished - 5 Feb 1993

Keywords

  • bacterial protein
  • hydrophobin
  • unclassified drug
  • article
  • Basidiomycetes
  • cell wall
  • fungus
  • hydrophobicity
  • nonhuman
  • priority journal

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