Influence of infection of cells with bacteria associated with reactive arthritis on the peptide repertoire presented by HLA-B27

J H Ringrose, A O Muijsers, Y. Pannekoek, B A Yard, C J Boog, L van Alphen, J. Dankert, Theodorus E W Feltkamp

Research output: Contribution to journalArticleAcademicpeer-review


Reactive arthritis (ReA) after infections with various gram-negative bacteria is strongly associated with the MHC class I molecule HLA-B27. It is supposed that the B27 molecule itself plays a role in the pathogenesis of ReA by presenting antigenic peptides to cytotoxic T lymphocytes. The peptide repertoires presented by Salmonella-, Shigella- and non-infected cells were compared to identify such peptides. From the peptides isolated from the B27 molecules of these cells, profiles were generated by reversed-phase chromatography and peaks present in the profiles from infected cells but not in profiles from non-infected cells were studied for their peptide compositions. Some sequences with identity to those in human histone H3, human ribosomal protein S17 and the heavy chain of HLA-B27 itself were detected only in profiles from infected cells. All peptides identified from infected cells contained the B*2705 peptide-binding motif. The data suggest that HLA-B27-positive cells infected with ReA-inducing bacteria show an increased presentation of certain self-peptides. There was no evidence for altered peptide-binding specificity of B27 after infection. However, the interpretations were hampered by the variation in peptide presentation between different experiments.

Original languageEnglish
Pages (from-to)385-9
Number of pages5
JournalJournal of Medical Microbiology
Issue number4
Publication statusPublished - Apr 2001
Externally publishedYes


  • Antigen Presentation
  • Arthritis, Reactive
  • Cells, Cultured
  • Dysentery, Bacillary
  • HLA-B27 Antigen
  • Humans
  • Peptides
  • Salmonella Infections
  • Salmonella typhimurium
  • Shigella flexneri
  • Journal Article
  • Research Support, Non-U.S. Gov't


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