Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants

Hesham Amin; Tina Šantl-Temkiv; Christine Cramer; Kai Finster; Francisco Gomez Real; Thorarinn Gislason; Mathias Holm; Christer Janson; Nils Oskar Jögi; Rain Jogi; Andrei Malinovschi; Ian P G Marshall; Lars Modig; Dan Norbäck; Rajesh Shigdel; Torben Sigsgaard; Cecilie Svanes; Hulda Thorarinsdottir; Inge M Wouters; Vivi Schlünssen; Randi J Bertelsen

doi:10.1021/acs.est.3c01616

Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants

Hesham Amin, Tina Šantl-Temkiv, Christine Cramer, Kai Finster, Francisco Gomez Real, Thorarinn Gislason, Mathias Holm, Christer Janson, Nils Oskar Jögi, Rain Jogi, Andrei Malinovschi, Ian P G Marshall, Lars Modig, Dan Norbäck, Rajesh Shigdel, Torben Sigsgaard, Cecilie Svanes, Hulda Thorarinsdottir, Inge M Wouters, Vivi SchlünssenRandi J Bertelsen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Airborne bacteria and endotoxin may affect asthma and allergies. However, there is limited understanding of the environmental determinants that influence them. This study investigated the airborne microbiomes in the homes of 1038 participants from five cities in Northern Europe: Aarhus, Bergen, Reykjavik, Tartu, and Uppsala. Airborne dust particles were sampled with electrostatic dust fall collectors (EDCs) from the participants' bedrooms. The dust washed from the EDCs' clothes was used to extract DNA and endotoxin. The DNA extracts were used for quantitative polymerase chain (qPCR) measurement and 16S rRNA gene sequencing, while endotoxin was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay. The results showed that households in Tartu and Aarhus had a higher bacterial load and diversity than those in Bergen and Reykjavik, possibly due to elevated concentrations of outdoor bacterial taxa associated with low precipitation and high wind speeds. Bergen-Tartu had the highest difference (ANOSIM R = 0.203) in β diversity. Multivariate regression models showed that α diversity indices and bacterial and endotoxin loads were positively associated with the occupants' age, number of occupants, cleaning frequency, presence of dogs, and age of the house. Further studies are needed to understand how meteorological factors influence the indoor bacterial community in light of climate change.

Original language	English
Pages (from-to)	11750–11766
Number of pages	17
Journal	Environmental Science & Technology
Volume	57
Issue number	32
Early online date	31 Jul 2023
DOIs	https://doi.org/10.1021/acs.est.3c01616
Publication status	Published - 15 Aug 2023

Bibliographical note

Funding Information:
This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 804199). K.F. acknowledges support by the Novo Nordisk Foundation (grant NNF19OC0056963), and T.Š.-T. acknowledges support by the Independent Research Fund Denmark (9145-00001B) and the Villum Fonden (research grants 23175 and 37435).

Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.

Keywords

Northern Europe
airborne microbiome
meteorological data
16S rRNA and occupants’ age

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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acs.est.3c01616Final published version, 12.3 MBLicence: CC BY

Cite this

Amin, H., Šantl-Temkiv, T., Cramer, C., Finster, K., Real, F. G., Gislason, T., Holm, M., Janson, C., Jögi, N. O., Jogi, R., Malinovschi, A., Marshall, I. P. G., Modig, L., Norbäck, D., Shigdel, R., Sigsgaard, T., Svanes, C., Thorarinsdottir, H., Wouters, I. M., ... Bertelsen, R. J. (2023). Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants. Environmental Science & Technology, 57(32), 11750–11766. https://doi.org/10.1021/acs.est.3c01616

@article{259d2c505e0549a296aaebf3596c7657,

title = "Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants",

abstract = "Airborne bacteria and endotoxin may affect asthma and allergies. However, there is limited understanding of the environmental determinants that influence them. This study investigated the airborne microbiomes in the homes of 1038 participants from five cities in Northern Europe: Aarhus, Bergen, Reykjavik, Tartu, and Uppsala. Airborne dust particles were sampled with electrostatic dust fall collectors (EDCs) from the participants' bedrooms. The dust washed from the EDCs' clothes was used to extract DNA and endotoxin. The DNA extracts were used for quantitative polymerase chain (qPCR) measurement and 16S rRNA gene sequencing, while endotoxin was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay. The results showed that households in Tartu and Aarhus had a higher bacterial load and diversity than those in Bergen and Reykjavik, possibly due to elevated concentrations of outdoor bacterial taxa associated with low precipitation and high wind speeds. Bergen-Tartu had the highest difference (ANOSIM R = 0.203) in β diversity. Multivariate regression models showed that α diversity indices and bacterial and endotoxin loads were positively associated with the occupants' age, number of occupants, cleaning frequency, presence of dogs, and age of the house. Further studies are needed to understand how meteorological factors influence the indoor bacterial community in light of climate change. ",

keywords = "Northern Europe, airborne microbiome, meteorological data, 16S rRNA and occupants{\textquoteright} age",

author = "Hesham Amin and Tina {\v S}antl-Temkiv and Christine Cramer and Kai Finster and Real, {Francisco Gomez} and Thorarinn Gislason and Mathias Holm and Christer Janson and J{\"o}gi, {Nils Oskar} and Rain Jogi and Andrei Malinovschi and Marshall, {Ian P G} and Lars Modig and Dan Norb{\"a}ck and Rajesh Shigdel and Torben Sigsgaard and Cecilie Svanes and Hulda Thorarinsdottir and Wouters, {Inge M} and Vivi Schl{\"u}nssen and Bertelsen, {Randi J}",

note = "Funding Information: This project has received funding from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement no. 804199). K.F. acknowledges support by the Novo Nordisk Foundation (grant NNF19OC0056963), and T.{\v S}.-T. acknowledges support by the Independent Research Fund Denmark (9145-00001B) and the Villum Fonden (research grants 23175 and 37435). Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

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doi = "10.1021/acs.est.3c01616",

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Amin, H, Šantl-Temkiv, T, Cramer, C, Finster, K, Real, FG, Gislason, T, Holm, M, Janson, C, Jögi, NO, Jogi, R, Malinovschi, A, Marshall, IPG, Modig, L, Norbäck, D, Shigdel, R, Sigsgaard, T, Svanes, C, Thorarinsdottir, H, Wouters, IM, Schlünssen, V & Bertelsen, RJ 2023, 'Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants', Environmental Science & Technology, vol. 57, no. 32, pp. 11750–11766. https://doi.org/10.1021/acs.est.3c01616

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T1 - Indoor Airborne Microbiome and Endotoxin

T2 - Meteorological Events and Occupant Characteristics Are Important Determinants

AU - Amin, Hesham

AU - Šantl-Temkiv, Tina

AU - Cramer, Christine

AU - Finster, Kai

AU - Real, Francisco Gomez

AU - Gislason, Thorarinn

AU - Holm, Mathias

AU - Janson, Christer

AU - Jögi, Nils Oskar

AU - Jogi, Rain

AU - Malinovschi, Andrei

AU - Marshall, Ian P G

AU - Modig, Lars

AU - Norbäck, Dan

AU - Shigdel, Rajesh

AU - Sigsgaard, Torben

AU - Svanes, Cecilie

AU - Thorarinsdottir, Hulda

AU - Wouters, Inge M

AU - Schlünssen, Vivi

AU - Bertelsen, Randi J

N1 - Funding Information: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 804199). K.F. acknowledges support by the Novo Nordisk Foundation (grant NNF19OC0056963), and T.Š.-T. acknowledges support by the Independent Research Fund Denmark (9145-00001B) and the Villum Fonden (research grants 23175 and 37435). Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/8/15

Y1 - 2023/8/15

N2 - Airborne bacteria and endotoxin may affect asthma and allergies. However, there is limited understanding of the environmental determinants that influence them. This study investigated the airborne microbiomes in the homes of 1038 participants from five cities in Northern Europe: Aarhus, Bergen, Reykjavik, Tartu, and Uppsala. Airborne dust particles were sampled with electrostatic dust fall collectors (EDCs) from the participants' bedrooms. The dust washed from the EDCs' clothes was used to extract DNA and endotoxin. The DNA extracts were used for quantitative polymerase chain (qPCR) measurement and 16S rRNA gene sequencing, while endotoxin was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay. The results showed that households in Tartu and Aarhus had a higher bacterial load and diversity than those in Bergen and Reykjavik, possibly due to elevated concentrations of outdoor bacterial taxa associated with low precipitation and high wind speeds. Bergen-Tartu had the highest difference (ANOSIM R = 0.203) in β diversity. Multivariate regression models showed that α diversity indices and bacterial and endotoxin loads were positively associated with the occupants' age, number of occupants, cleaning frequency, presence of dogs, and age of the house. Further studies are needed to understand how meteorological factors influence the indoor bacterial community in light of climate change.

AB - Airborne bacteria and endotoxin may affect asthma and allergies. However, there is limited understanding of the environmental determinants that influence them. This study investigated the airborne microbiomes in the homes of 1038 participants from five cities in Northern Europe: Aarhus, Bergen, Reykjavik, Tartu, and Uppsala. Airborne dust particles were sampled with electrostatic dust fall collectors (EDCs) from the participants' bedrooms. The dust washed from the EDCs' clothes was used to extract DNA and endotoxin. The DNA extracts were used for quantitative polymerase chain (qPCR) measurement and 16S rRNA gene sequencing, while endotoxin was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay. The results showed that households in Tartu and Aarhus had a higher bacterial load and diversity than those in Bergen and Reykjavik, possibly due to elevated concentrations of outdoor bacterial taxa associated with low precipitation and high wind speeds. Bergen-Tartu had the highest difference (ANOSIM R = 0.203) in β diversity. Multivariate regression models showed that α diversity indices and bacterial and endotoxin loads were positively associated with the occupants' age, number of occupants, cleaning frequency, presence of dogs, and age of the house. Further studies are needed to understand how meteorological factors influence the indoor bacterial community in light of climate change.

KW - Northern Europe

KW - airborne microbiome

KW - meteorological data

KW - 16S rRNA and occupants’ age

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U2 - 10.1021/acs.est.3c01616

DO - 10.1021/acs.est.3c01616

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SN - 0013-936X

VL - 57

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JO - Environmental Science & Technology

JF - Environmental Science & Technology

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ER -

Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants

Abstract

Bibliographical note

Keywords

UN SDGs

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