Indirect reduction of Ralstonia solanacearum via pathogen helper inhibition

Mei Li; Thomas Pommier; Yue Yin; Jianing Wang; Shaohua Gu; Alexandre Jousset; Joost Keuskamp; Honggui Wang; Zhong Wei; Yangchun Xu; Qirong Shen; George A. Kowalchuk

doi:10.1038/s41396-021-01126-2

Indirect reduction of Ralstonia solanacearum via pathogen helper inhibition

Mei Li
, Thomas Pommier
, Yue Yin
, Jianing Wang
, Shaohua Gu
, Alexandre Jousset
, Joost Keuskamp
, Honggui Wang
, Zhong Wei^*
, Yangchun Xu
, Qirong Shen
, George A. Kowalchuk

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The rhizosphere microbiome forms a first line of defense against soilborne pathogens. To date, most microbiome enhancement strategies have relied on bioaugmentation with antagonistic microorganisms that directly inhibit pathogens. Previous studies have shown that some root-associated bacteria are able to facilitate pathogen growth. We therefore hypothesized that inhibiting such pathogen helpers may help reduce pathogen densities. We examined tripartite interactions between a model pathogen, Ralstonia solanacearum, two model helper strains and a collection of 46 bacterial isolates recovered from the tomato rhizosphere. This system allowed us to examine the importance of direct (effects of rhizobacteria on pathogen growth) and indirect (effects of rhizobacteria on helper growth) pathways affecting pathogen growth. We found that the interaction between rhizosphere isolates and the helper strains was the major determinant of pathogen suppression both in vitro and in vivo. We therefore propose that controlling microbiome composition to prevent the growth of pathogen helpers may become part of sustainable strategies for pathogen control.

Original language	English
Pages (from-to)	868-875
Number of pages	8
Journal	ISME Journal
Volume	16
Issue number	3
Early online date	20 Oct 2021
DOIs	https://doi.org/10.1038/s41396-021-01126-2
Publication status	Published - Mar 2022

Bibliographical note

Funding Information:
This research was financially supported by the National Key Research and Development Program of China (SQ2021YFD1900024), National Natural Science Foundation of China (31972504, 42090060, 41922053, and 42007038), the Fundamental Research Funds for the Central Universities (KY2201719, KYT201802, KYXK202010, KJQN202116-KJQN202117), the Natural Science Foundation of Jiangsu Province (BK20190518, BK20180527 and BK20200533), and technically supported by the Bioinformatics Center of Nanjing Agricultural University. ML was supported by Chinese Scholarship Council (CSC).

Publisher Copyright:
© 2021, The Author(s).

Funding

This research was financially supported by the National Key Research and Development Program of China (SQ2021YFD1900024), National Natural Science Foundation of China (31972504, 42090060, 41922053, and 42007038), the Fundamental Research Funds for the Central Universities (KY2201719, KYT201802, KYXK202010, KJQN202116-KJQN202117), the Natural Science Foundation of Jiangsu Province (BK20190518, BK20180527 and BK20200533), and technically supported by the Bioinformatics Center of Nanjing Agricultural University. ML was supported by Chinese Scholarship Council (CSC).

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Cite this

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title = "Indirect reduction of Ralstonia solanacearum via pathogen helper inhibition",

abstract = "The rhizosphere microbiome forms a first line of defense against soilborne pathogens. To date, most microbiome enhancement strategies have relied on bioaugmentation with antagonistic microorganisms that directly inhibit pathogens. Previous studies have shown that some root-associated bacteria are able to facilitate pathogen growth. We therefore hypothesized that inhibiting such pathogen helpers may help reduce pathogen densities. We examined tripartite interactions between a model pathogen, Ralstonia solanacearum, two model helper strains and a collection of 46 bacterial isolates recovered from the tomato rhizosphere. This system allowed us to examine the importance of direct (effects of rhizobacteria on pathogen growth) and indirect (effects of rhizobacteria on helper growth) pathways affecting pathogen growth. We found that the interaction between rhizosphere isolates and the helper strains was the major determinant of pathogen suppression both in vitro and in vivo. We therefore propose that controlling microbiome composition to prevent the growth of pathogen helpers may become part of sustainable strategies for pathogen control.",

author = "Mei Li and Thomas Pommier and Yue Yin and Jianing Wang and Shaohua Gu and Alexandre Jousset and Joost Keuskamp and Honggui Wang and Zhong Wei and Yangchun Xu and Qirong Shen and Kowalchuk, \{George A.\}",

note = "Funding Information: This research was financially supported by the National Key Research and Development Program of China (SQ2021YFD1900024), National Natural Science Foundation of China (31972504, 42090060, 41922053, and 42007038), the Fundamental Research Funds for the Central Universities (KY2201719, KYT201802, KYXK202010, KJQN202116-KJQN202117), the Natural Science Foundation of Jiangsu Province (BK20190518, BK20180527 and BK20200533), and technically supported by the Bioinformatics Center of Nanjing Agricultural University. ML was supported by Chinese Scholarship Council (CSC). Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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AU - Keuskamp, Joost

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AU - Shen, Qirong

AU - Kowalchuk, George A.

N1 - Funding Information: This research was financially supported by the National Key Research and Development Program of China (SQ2021YFD1900024), National Natural Science Foundation of China (31972504, 42090060, 41922053, and 42007038), the Fundamental Research Funds for the Central Universities (KY2201719, KYT201802, KYXK202010, KJQN202116-KJQN202117), the Natural Science Foundation of Jiangsu Province (BK20190518, BK20180527 and BK20200533), and technically supported by the Bioinformatics Center of Nanjing Agricultural University. ML was supported by Chinese Scholarship Council (CSC). Publisher Copyright: © 2021, The Author(s).

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N2 - The rhizosphere microbiome forms a first line of defense against soilborne pathogens. To date, most microbiome enhancement strategies have relied on bioaugmentation with antagonistic microorganisms that directly inhibit pathogens. Previous studies have shown that some root-associated bacteria are able to facilitate pathogen growth. We therefore hypothesized that inhibiting such pathogen helpers may help reduce pathogen densities. We examined tripartite interactions between a model pathogen, Ralstonia solanacearum, two model helper strains and a collection of 46 bacterial isolates recovered from the tomato rhizosphere. This system allowed us to examine the importance of direct (effects of rhizobacteria on pathogen growth) and indirect (effects of rhizobacteria on helper growth) pathways affecting pathogen growth. We found that the interaction between rhizosphere isolates and the helper strains was the major determinant of pathogen suppression both in vitro and in vivo. We therefore propose that controlling microbiome composition to prevent the growth of pathogen helpers may become part of sustainable strategies for pathogen control.

AB - The rhizosphere microbiome forms a first line of defense against soilborne pathogens. To date, most microbiome enhancement strategies have relied on bioaugmentation with antagonistic microorganisms that directly inhibit pathogens. Previous studies have shown that some root-associated bacteria are able to facilitate pathogen growth. We therefore hypothesized that inhibiting such pathogen helpers may help reduce pathogen densities. We examined tripartite interactions between a model pathogen, Ralstonia solanacearum, two model helper strains and a collection of 46 bacterial isolates recovered from the tomato rhizosphere. This system allowed us to examine the importance of direct (effects of rhizobacteria on pathogen growth) and indirect (effects of rhizobacteria on helper growth) pathways affecting pathogen growth. We found that the interaction between rhizosphere isolates and the helper strains was the major determinant of pathogen suppression both in vitro and in vivo. We therefore propose that controlling microbiome composition to prevent the growth of pathogen helpers may become part of sustainable strategies for pathogen control.

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Indirect reduction of Ralstonia solanacearum via pathogen helper inhibition

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