Abstract
To harmoniously coordinate the activities of all its different cell types, a multicellular organism critically depends on intercellular communication. One recently discovered mode of intercellular cross-talk is based on the exchange of "extracellular vesicles" (EVs). EVs are nano-sized heterogeneous lipid bilayer vesicles enriched in a variety of biomolecules that mediate short- and long-distance communication between different cells, and between cells and their environment. Numerous studies have demonstrated important aspects pertaining to the dynamics of their release, their uptake, and sub-cellular fate and roles in vitro. However, to demonstrate these and other aspects of EV biology in a relevant, fully physiological context in vivo remains challenging. In this review we analyze the state of the art of EV imaging in vivo, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real-time and expand our understanding of EV biology at cellular and systems level.
Original language | English |
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Pages (from-to) | 918-929 |
Number of pages | 12 |
Journal | FASEB BioAdvances |
Volume | 3 |
Issue number | 11 |
Early online date | 9 Aug 2021 |
DOIs | |
Publication status | Published - Nov 2021 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was funded by Horizon 2020—Marie Skłodowska‐Curie Actions—Innovative Training Network (H2020‐MSCA‐ITN) “” to VV, grants from the National Institutes of Health (NIH) and the Agence Nationale de la Recherche (ANR) to AB, and Institut National du Cancer‐projets libres “Biologie et Sciences du Cancer”’ (INCa‐PLBIO) to FJV. proEVLifeCycle
Funding Information:
This work was funded by Horizon 2020?Marie Sk?odowska-Curie Actions?Innovative Training Network (H2020-MSCA-ITN) ?proEVLifeCycle? to VV, grants from the National Institutes of Health (NIH) and the Agence Nationale de la Recherche (ANR) to AB, and Institut National du Cancer-projets libres ?Biologie et Sciences du Cancer?? (INCa-PLBIO) to FJV.
Publisher Copyright:
© 2021 The Authors. FASEB BioAdvances published by The Federation of American Societies for Experimental Biology.
Keywords
- exosomes
- extracellular vesicles
- homeostasis
- live-imaging
- zebrafish