In vivo imaging of EVs in zebrafish: New perspectives from “the waterside”

Vincenzo Verdi, Anaïs Bécot, Guillaume Niel, Frederik J. Verweij

Research output: Contribution to journalReview articlepeer-review

Abstract

To harmoniously coordinate the activities of all its different cell types, a multicellular organism critically depends on intercellular communication. One recently discovered mode of intercellular cross-talk is based on the exchange of "extracellular vesicles" (EVs). EVs are nano-sized heterogeneous lipid bilayer vesicles enriched in a variety of biomolecules that mediate short- and long-distance communication between different cells, and between cells and their environment. Numerous studies have demonstrated important aspects pertaining to the dynamics of their release, their uptake, and sub-cellular fate and roles in vitro. However, to demonstrate these and other aspects of EV biology in a relevant, fully physiological context in vivo remains challenging. In this review we analyze the state of the art of EV imaging in vivo, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real-time and expand our understanding of EV biology at cellular and systems level.
Original languageEnglish
Pages (from-to)918-929
Number of pages12
JournalFASEB BioAdvances
Volume3
Issue number11
Early online date9 Aug 2021
DOIs
Publication statusPublished - Nov 2021
Externally publishedYes

Bibliographical note

Funding Information:
This work was funded by Horizon 2020—Marie Skłodowska‐Curie Actions—Innovative Training Network (H2020‐MSCA‐ITN) “” to VV, grants from the National Institutes of Health (NIH) and the Agence Nationale de la Recherche (ANR) to AB, and Institut National du Cancer‐projets libres “Biologie et Sciences du Cancer”’ (INCa‐PLBIO) to FJV. proEVLifeCycle

Funding Information:
This work was funded by Horizon 2020?Marie Sk?odowska-Curie Actions?Innovative Training Network (H2020-MSCA-ITN) ?proEVLifeCycle? to VV, grants from the National Institutes of Health (NIH) and the Agence Nationale de la Recherche (ANR) to AB, and Institut National du Cancer-projets libres ?Biologie et Sciences du Cancer?? (INCa-PLBIO) to FJV.

Publisher Copyright:
© 2021 The Authors. FASEB BioAdvances published by The Federation of American Societies for Experimental Biology.

Keywords

  • exosomes
  • extracellular vesicles
  • homeostasis
  • live-imaging
  • zebrafish

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