In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein

Rogier Bodewes; Martina M Geelhoed-Mieras; Jens Wrammert; Rafi Ahmed; Patrick C Wilson; Ron A M Fouchier; Albert D M E Osterhaus; Guus F Rimmelzwaan

doi:10.1128/CVI.00339-13

In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein

Rogier Bodewes, Martina M Geelhoed-Mieras, Jens Wrammert, Rafi Ahmed, Patrick C Wilson, Ron A M Fouchier, Albert D M E Osterhaus, Guus F Rimmelzwaan

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Abstract

Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.

Original language	English
Pages (from-to)	1333-7
Number of pages	5
Journal	Clinical and Vaccine Immunology
Volume	20
Issue number	8
DOIs	https://doi.org/10.1128/CVI.00339-13
Publication status	Published - Aug 2013

Keywords

Antibodies, Monoclonal
Antibodies, Viral
Antigens, Viral
Humans
Influenza A virus
Protein Binding
RNA-Binding Proteins
T-Lymphocytes
Viral Core Proteins

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10.1128/CVI.00339-13

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title = "In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein",

abstract = "Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.",

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doi = "10.1128/CVI.00339-13",

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T1 - In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein

AU - Bodewes, Rogier

AU - Geelhoed-Mieras, Martina M

AU - Wrammert, Jens

AU - Ahmed, Rafi

AU - Wilson, Patrick C

AU - Fouchier, Ron A M

AU - Osterhaus, Albert D M E

AU - Rimmelzwaan, Guus F

PY - 2013/8

Y1 - 2013/8

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AB - Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.

KW - Antibodies, Monoclonal

KW - Antibodies, Viral

KW - Antigens, Viral

KW - Humans

KW - Influenza A virus

KW - Protein Binding

KW - RNA-Binding Proteins

KW - T-Lymphocytes

KW - Viral Core Proteins

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DO - 10.1128/CVI.00339-13

M3 - Article

C2 - 23761662

SN - 1556-6811

VL - 20

SP - 1333

EP - 1337

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 8

ER -

In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein

Abstract

Keywords

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