TY - JOUR
T1 - In situ Delivery of Antigen to DC-SIGN + CD14 + Dermal Dendritic Cells Results in Enhanced CD8 + T-Cell Responses
AU - Fehres, Cynthia M.
AU - Van Beelen, Astrid J.
AU - Bruijns, Sven C M
AU - Ambrosini, Martino
AU - Kalay, Hakan
AU - Van Bloois, Louis
AU - Unger, Wendy W J
AU - Garcia-Vallejo, Juan J.
AU - Storm, G
AU - De Gruijl, Tanja D.
AU - Van Kooyk, Yvette V.
PY - 2015/9/18
Y1 - 2015/9/18
N2 - CD14 + dendritic cells (DCs) present in the dermis of human skin represent a large subset of dermal DCs (dDCs) that are considered macrophage-like cells with poor antigen (cross)-presenting capacity and limited migratory potential to the lymph nodes. CD14 + dDC highly express DC-specific ICAM-3-grabbing non-integrin (DC-SIGN), a receptor containing potent endocytic capacity, facilitating intracellular routing of antigens to major histocompatibility complex I and II (MHC-I andII) loading compartments for the presentation to antigen-specific CD8 + and CD4 + T cells. Here we show using a human skin explant model that the in situ targeting of antigens to DC-SIGN using glycan-modified liposomes enhances the antigen-presenting capacity of CD14 + dDCs. Intradermal vaccination of liposomes modified with the DC-SIGN-targeting glycan Lewis X, containing melanoma antigens (MART-1 or Gp100), accumulated in CD14 + dDCs and resulted in enhanced Gp100- or MART-1-specific CD8 + T-cell responses. Simultaneous intradermal injection of the cytokines GM-CSF and IL-4 as adjuvant enhanced the migration of the skin DCs and increased the expression of DC-SIGN on the CD14 + and CD1a + dDCs. These data demonstrate that human CD14 + dDCs exhibit potent cross-presenting capacity when targeted in situ through DC-SIGN.
AB - CD14 + dendritic cells (DCs) present in the dermis of human skin represent a large subset of dermal DCs (dDCs) that are considered macrophage-like cells with poor antigen (cross)-presenting capacity and limited migratory potential to the lymph nodes. CD14 + dDC highly express DC-specific ICAM-3-grabbing non-integrin (DC-SIGN), a receptor containing potent endocytic capacity, facilitating intracellular routing of antigens to major histocompatibility complex I and II (MHC-I andII) loading compartments for the presentation to antigen-specific CD8 + and CD4 + T cells. Here we show using a human skin explant model that the in situ targeting of antigens to DC-SIGN using glycan-modified liposomes enhances the antigen-presenting capacity of CD14 + dDCs. Intradermal vaccination of liposomes modified with the DC-SIGN-targeting glycan Lewis X, containing melanoma antigens (MART-1 or Gp100), accumulated in CD14 + dDCs and resulted in enhanced Gp100- or MART-1-specific CD8 + T-cell responses. Simultaneous intradermal injection of the cytokines GM-CSF and IL-4 as adjuvant enhanced the migration of the skin DCs and increased the expression of DC-SIGN on the CD14 + and CD1a + dDCs. These data demonstrate that human CD14 + dDCs exhibit potent cross-presenting capacity when targeted in situ through DC-SIGN.
UR - http://www.scopus.com/inward/record.url?scp=84939469470&partnerID=8YFLogxK
U2 - 10.1038/jid.2015.152
DO - 10.1038/jid.2015.152
M3 - Article
C2 - 25885805
AN - SCOPUS:84939469470
SN - 0022-202X
VL - 135
SP - 2228
EP - 2236
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 9
ER -