In situ Delivery of Antigen to DC-SIGN + CD14 + Dermal Dendritic Cells Results in Enhanced CD8 + T-Cell Responses

Cynthia M. Fehres, Astrid J. Van Beelen, Sven C M Bruijns, Martino Ambrosini, Hakan Kalay, Louis Van Bloois, Wendy W J Unger, Juan J. Garcia-Vallejo, G Storm, Tanja D. De Gruijl, Yvette V. Van Kooyk*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

CD14 + dendritic cells (DCs) present in the dermis of human skin represent a large subset of dermal DCs (dDCs) that are considered macrophage-like cells with poor antigen (cross)-presenting capacity and limited migratory potential to the lymph nodes. CD14 + dDC highly express DC-specific ICAM-3-grabbing non-integrin (DC-SIGN), a receptor containing potent endocytic capacity, facilitating intracellular routing of antigens to major histocompatibility complex I and II (MHC-I andII) loading compartments for the presentation to antigen-specific CD8 + and CD4 + T cells. Here we show using a human skin explant model that the in situ targeting of antigens to DC-SIGN using glycan-modified liposomes enhances the antigen-presenting capacity of CD14 + dDCs. Intradermal vaccination of liposomes modified with the DC-SIGN-targeting glycan Lewis X, containing melanoma antigens (MART-1 or Gp100), accumulated in CD14 + dDCs and resulted in enhanced Gp100- or MART-1-specific CD8 + T-cell responses. Simultaneous intradermal injection of the cytokines GM-CSF and IL-4 as adjuvant enhanced the migration of the skin DCs and increased the expression of DC-SIGN on the CD14 + and CD1a + dDCs. These data demonstrate that human CD14 + dDCs exhibit potent cross-presenting capacity when targeted in situ through DC-SIGN.

Original languageEnglish
Pages (from-to)2228-2236
Number of pages9
JournalJournal of Investigative Dermatology
Volume135
Issue number9
DOIs
Publication statusPublished - 18 Sept 2015

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