Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer

Astrid A Streng; Kirsten C G Van Dycke; Conny T M van Oostrom; Daniela C F Salvatori; Gerben Hulsegge; Inês Chaves; Till Roenneberg; Serge A L Zander; Harry van Steeg; Gijsbertus T J van der Horst; Linda W M van Kerkhof

doi:10.1177/07487304231178340

Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer

Astrid A Streng, Kirsten C G Van Dycke, Conny T M van Oostrom, Daniela C F Salvatori, Gerben Hulsegge, Inês Chaves, Till Roenneberg, Serge A L Zander, Harry van Steeg, Gijsbertus T J van der Horst, Linda W M van Kerkhof^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Epidemiological studies associate night shift work with increased breast cancer risk. However, the underlying mechanisms are not clearly understood. To better understand these mechanisms, animal models that mimic the human situation of different aspects of shift work are needed. In this study, we used "timed sleep restriction" (TSR) cages to simulate clockwise and counterclockwise rotating shift work schedules and investigated predicted sleep patterns and mammary tumor development in breast tumor-prone female p53R270H©/+WAPCre mice. We show that TSR cages are effective in disturbing normal activity and estimated sleep patterns. Although circadian rhythms were not shifted, we observed effects of the rotating schedules on sleep timing and sleep duration. Sleep loss during a simulated shift was partly compensated after the shift and also partly during the free days. No effects were observed on body weight gain and latency time of breast cancer development. In summary, our study shows that the TSR cages can be used to model shift work in mice and affect patterns of activity and sleep. The effect of disturbing sleep patterns on carcinogenesis needs to be further investigated.

Original language	English
Pages (from-to)	476-491
Number of pages	16
Journal	Journal of Biological Rhythms
Volume	38
Issue number	5
Early online date	26 Jun 2023
DOIs	https://doi.org/10.1177/07487304231178340
Publication status	Published - Oct 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s).

Funding

We thank Stefanie Vester for assistance with the mouse experiment and processing all the mouse tissues and preparing the slides for histopathological evaluation. We thank Dr. Martijn Dollé for providing valuable feedback on this manuscript. This study was funded by the SPR Program of the Dutch National Institute for Public Health and the Environment (RIVM) (grant S015012), the Dutch Ministry of Social Affairs and Employment (KV11.16), Worldwide Cancer Research (grant 16-1345), and the BioClock Consortium (an NWA-ORC program of the Dutch Research Council; project number 1292.19.077).

Funders	Funder number
BioClock Consortium
Dutch National Institute for Public Health and the Environment
NWA-ORC
Worldwide Cancer Research	16-1345
Ministerie van Sociale Zaken en Werkgelegenheid	KV11.16
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	1292.19.077
Rijksinstituut voor Volksgezondheid en Milieu	S015012

Keywords

breast cancer
circadian clock
circadian disturbance
shift work

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/07487304231178340

streng-et-al-2023-impact-of-simulated-rotating-shift-work-on-mammary-tumor-development-in-the-p53r270h-c-wapcre-mouseFinal published version, 3.69 MBLicence: Taverne

Cite this

Streng, A. A., Van Dycke, K. C. G., van Oostrom, C. T. M., Salvatori, D. C. F., Hulsegge, G., Chaves, I., Roenneberg, T., Zander, S. A. L., van Steeg, H., van der Horst, G. T. J., & van Kerkhof, L. W. M. (2023). Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer. Journal of Biological Rhythms, 38(5), 476-491. https://doi.org/10.1177/07487304231178340

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abstract = "Epidemiological studies associate night shift work with increased breast cancer risk. However, the underlying mechanisms are not clearly understood. To better understand these mechanisms, animal models that mimic the human situation of different aspects of shift work are needed. In this study, we used {"}timed sleep restriction{"} (TSR) cages to simulate clockwise and counterclockwise rotating shift work schedules and investigated predicted sleep patterns and mammary tumor development in breast tumor-prone female p53R270H{\textcopyright}/+WAPCre mice. We show that TSR cages are effective in disturbing normal activity and estimated sleep patterns. Although circadian rhythms were not shifted, we observed effects of the rotating schedules on sleep timing and sleep duration. Sleep loss during a simulated shift was partly compensated after the shift and also partly during the free days. No effects were observed on body weight gain and latency time of breast cancer development. In summary, our study shows that the TSR cages can be used to model shift work in mice and affect patterns of activity and sleep. The effect of disturbing sleep patterns on carcinogenesis needs to be further investigated.",

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Streng, AA, Van Dycke, KCG, van Oostrom, CTM, Salvatori, DCF, Hulsegge, G, Chaves, I, Roenneberg, T, Zander, SAL, van Steeg, H, van der Horst, GTJ & van Kerkhof, LWM 2023, 'Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer', Journal of Biological Rhythms, vol. 38, no. 5, pp. 476-491. https://doi.org/10.1177/07487304231178340

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AU - Streng, Astrid A

AU - Van Dycke, Kirsten C G

AU - van Oostrom, Conny T M

AU - Salvatori, Daniela C F

AU - Hulsegge, Gerben

AU - Chaves, Inês

AU - Roenneberg, Till

AU - Zander, Serge A L

AU - van Steeg, Harry

AU - van der Horst, Gijsbertus T J

AU - van Kerkhof, Linda W M

PY - 2023/10

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N2 - Epidemiological studies associate night shift work with increased breast cancer risk. However, the underlying mechanisms are not clearly understood. To better understand these mechanisms, animal models that mimic the human situation of different aspects of shift work are needed. In this study, we used "timed sleep restriction" (TSR) cages to simulate clockwise and counterclockwise rotating shift work schedules and investigated predicted sleep patterns and mammary tumor development in breast tumor-prone female p53R270H©/+WAPCre mice. We show that TSR cages are effective in disturbing normal activity and estimated sleep patterns. Although circadian rhythms were not shifted, we observed effects of the rotating schedules on sleep timing and sleep duration. Sleep loss during a simulated shift was partly compensated after the shift and also partly during the free days. No effects were observed on body weight gain and latency time of breast cancer development. In summary, our study shows that the TSR cages can be used to model shift work in mice and affect patterns of activity and sleep. The effect of disturbing sleep patterns on carcinogenesis needs to be further investigated.

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Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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