Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis

Corma Ma Otte; Chiara Valtolina; Sandra Vreman; Siobhan Hubers; Monique E van Wolferen; Robert P Favier; Jan Rothuizen; Louis C Penning

doi:10.1177/1098612X17699723

Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis

Corma Ma Otte, Chiara Valtolina, Sandra Vreman, Siobhan Hubers, Monique E van Wolferen, Robert P Favier, Jan Rothuizen, Louis C Penning

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objectives The aim of the study was to compare the hepatic progenitor cell niche in healthy feline livers and the liver tissue of cats with lymphocytic cholangitis. Methods Immunohistochemical stainings for vimentin, laminin, beta (β)-catenin and Notch1 intracellular domain (NICD) were used on formalin-fixed liver biopsies from affected (n = 12) and unaffected cats (n = 2). Results All immunohistochemical markers used were expressed in more cells, or more intensely, in the liver tissue of cats with lymphocytic cholangitis than in the liver tissue of unaffected cats. Conclusions and relevance Enhanced expression of vimentin, laminin, cytoplasmic/nuclear β-catenin and NICD in liver biopsies from cats with lymphocytic cholangitis indicates that the hepatic progenitor cell (HPC) niche is remodelled and activated. HPCs might provide insights into new regenerative treatment options for lymphocytic cholangitis in cats in the future.

Original language	English
Pages (from-to)	30-37
Number of pages	8
Journal	Journal of Feline Medicine and Surgery
Volume	20
Issue number	1
DOIs	https://doi.org/10.1177/1098612X17699723
Publication status	Published - Jan 2018

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title = "Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis",

abstract = "Objectives The aim of the study was to compare the hepatic progenitor cell niche in healthy feline livers and the liver tissue of cats with lymphocytic cholangitis. Methods Immunohistochemical stainings for vimentin, laminin, beta (β)-catenin and Notch1 intracellular domain (NICD) were used on formalin-fixed liver biopsies from affected (n = 12) and unaffected cats (n = 2). Results All immunohistochemical markers used were expressed in more cells, or more intensely, in the liver tissue of cats with lymphocytic cholangitis than in the liver tissue of unaffected cats. Conclusions and relevance Enhanced expression of vimentin, laminin, cytoplasmic/nuclear β-catenin and NICD in liver biopsies from cats with lymphocytic cholangitis indicates that the hepatic progenitor cell (HPC) niche is remodelled and activated. HPCs might provide insights into new regenerative treatment options for lymphocytic cholangitis in cats in the future.",

author = "Otte, {Corma Ma} and Chiara Valtolina and Sandra Vreman and Siobhan Hubers and {van Wolferen}, {Monique E} and Favier, {Robert P} and Jan Rothuizen and Penning, {Louis C}",

year = "2018",

month = jan,

doi = "10.1177/1098612X17699723",

language = "English",

volume = "20",

pages = "30--37",

journal = "Journal of Feline Medicine and Surgery",

issn = "1098-612X",

publisher = "SAGE Publications Inc.",

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T1 - Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis

AU - Otte, Corma Ma

AU - Valtolina, Chiara

AU - Vreman, Sandra

AU - Hubers, Siobhan

AU - van Wolferen, Monique E

AU - Favier, Robert P

AU - Rothuizen, Jan

AU - Penning, Louis C

PY - 2018/1

Y1 - 2018/1

N2 - Objectives The aim of the study was to compare the hepatic progenitor cell niche in healthy feline livers and the liver tissue of cats with lymphocytic cholangitis. Methods Immunohistochemical stainings for vimentin, laminin, beta (β)-catenin and Notch1 intracellular domain (NICD) were used on formalin-fixed liver biopsies from affected (n = 12) and unaffected cats (n = 2). Results All immunohistochemical markers used were expressed in more cells, or more intensely, in the liver tissue of cats with lymphocytic cholangitis than in the liver tissue of unaffected cats. Conclusions and relevance Enhanced expression of vimentin, laminin, cytoplasmic/nuclear β-catenin and NICD in liver biopsies from cats with lymphocytic cholangitis indicates that the hepatic progenitor cell (HPC) niche is remodelled and activated. HPCs might provide insights into new regenerative treatment options for lymphocytic cholangitis in cats in the future.

AB - Objectives The aim of the study was to compare the hepatic progenitor cell niche in healthy feline livers and the liver tissue of cats with lymphocytic cholangitis. Methods Immunohistochemical stainings for vimentin, laminin, beta (β)-catenin and Notch1 intracellular domain (NICD) were used on formalin-fixed liver biopsies from affected (n = 12) and unaffected cats (n = 2). Results All immunohistochemical markers used were expressed in more cells, or more intensely, in the liver tissue of cats with lymphocytic cholangitis than in the liver tissue of unaffected cats. Conclusions and relevance Enhanced expression of vimentin, laminin, cytoplasmic/nuclear β-catenin and NICD in liver biopsies from cats with lymphocytic cholangitis indicates that the hepatic progenitor cell (HPC) niche is remodelled and activated. HPCs might provide insights into new regenerative treatment options for lymphocytic cholangitis in cats in the future.

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DO - 10.1177/1098612X17699723

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SN - 1098-612X

VL - 20

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EP - 37

JO - Journal of Feline Medicine and Surgery

JF - Journal of Feline Medicine and Surgery

IS - 1

ER -

Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis

Abstract

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