TY - JOUR
T1 - Frigoriglobus tundricola gen. nov., sp. nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland
AU - Kulichevskaya, Irina S.
AU - Ivanova, Anastasia A.
AU - Naumoff, Daniil G.
AU - Beletsky, Alexey
AU - Rijpstra, W. Irene C.
AU - Damste, Jaap S. Sinninghe
AU - Mardanov, Andrey
AU - Ravin, Nikolai
AU - Dedysh, Svetlana N.
N1 - Cited By :20
Export Date: 24 November 2023
PY - 2020/9/5
Y1 - 2020/9/5
N2 - Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood. In this study, we characterized a novel representative of this family, strain PL17(T), which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose. Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates. Strain PL17(T) was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures. The major fatty acids were C-18:0, C-16:1 omega 5, and beta OH-C-16:1; the major polar lipid was trimethylornithine. The genome of strain PL17(T) consisted of a 9.83 Mb chromosome and a 24.69 kb plasmid. The G + C contents of the chromosomal and plasmid DNA were 67.4 and 62.3 mol%, respectively. Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases. The genome of strain PL17(T) contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246(T). Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17(T) (= CECT 9407(T) =VKM B-3467(T)) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp. nov. (C) 2020 The Author(s). Published by Elsevier GmbH.
AB - Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood. In this study, we characterized a novel representative of this family, strain PL17(T), which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose. Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates. Strain PL17(T) was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures. The major fatty acids were C-18:0, C-16:1 omega 5, and beta OH-C-16:1; the major polar lipid was trimethylornithine. The genome of strain PL17(T) consisted of a 9.83 Mb chromosome and a 24.69 kb plasmid. The G + C contents of the chromosomal and plasmid DNA were 67.4 and 62.3 mol%, respectively. Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases. The genome of strain PL17(T) contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246(T). Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17(T) (= CECT 9407(T) =VKM B-3467(T)) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp. nov. (C) 2020 The Author(s). Published by Elsevier GmbH.
KW - Cellulose utilization
KW - Frigoriglobus tundricola gen. nov., sp. nov.
KW - Large genome
KW - Planctomycetes
KW - Psychrotolerance
KW - Unlinked rRNA operon
U2 - 10.1016/j.syapm.2020.126129
DO - 10.1016/j.syapm.2020.126129
M3 - Article
C2 - 32847778
SN - 0723-2020
VL - 43
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 5
M1 - 126129
ER -