Identification of monoclonal antibodies with specificity to α- or β-chains of β2-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle

V. P.M.G. Rutten; A. Hoek; K. E. Müller

doi:10.1016/0165-2427(96)05585-7

Identification of monoclonal antibodies with specificity to α- or β-chains of β₂-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle

V. P.M.G. Rutten^*, A. Hoek, K. E. Müller

^*Corresponding author for this work

Immunology

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The reactivity of monoclonal antibodies entered in the panels of the Third Workshop on Ruminant Leucocyte Antigens with lymphocytes and monocytes of normal and β₂-integrin deficient (Bovine Leucocyte Adhesion Deficiency: BLAD) animals was determined by flow cytometry to investigate potential specificities to α- or β-chains of β₂-integrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but three had reactivity with cells from BLAD animals. We conclude that our approach provides an easy way to reliably identify the majority of β₂-integrin specific monoclonal antibodies.

Original language	English
Pages (from-to)	341-345
Number of pages	5
Journal	Veterinary Immunology and Immunopathology
Volume	52
Issue number	4
DOIs	https://doi.org/10.1016/0165-2427(96)05585-7
Publication status	Published - Aug 1996

Keywords

BLAD
Cattle
Leucocytes
Monoclonal antibodies

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10.1016/0165-2427(96)05585-7

Cite this

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title = "Identification of monoclonal antibodies with specificity to α- or β-chains of β2-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle",

abstract = "The reactivity of monoclonal antibodies entered in the panels of the Third Workshop on Ruminant Leucocyte Antigens with lymphocytes and monocytes of normal and β2-integrin deficient (Bovine Leucocyte Adhesion Deficiency: BLAD) animals was determined by flow cytometry to investigate potential specificities to α- or β-chains of β2-integrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but three had reactivity with cells from BLAD animals. We conclude that our approach provides an easy way to reliably identify the majority of β2-integrin specific monoclonal antibodies.",

keywords = "BLAD, Cattle, Leucocytes, Monoclonal antibodies",

author = "Rutten, \{V. P.M.G.\} and A. Hoek and M{\"u}ller, \{K. E.\}",

year = "1996",

month = aug,

doi = "10.1016/0165-2427(96)05585-7",

language = "English",

volume = "52",

pages = "341--345",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier BV",

number = "4",

}

Identification of monoclonal antibodies with specificity to α- or β-chains of β₂-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle. / Rutten, V. P.M.G.; Hoek, A.; Müller, K. E.
In: Veterinary Immunology and Immunopathology, Vol. 52, No. 4, 08.1996, p. 341-345.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Identification of monoclonal antibodies with specificity to α- or β-chains of β2-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle

AU - Rutten, V. P.M.G.

AU - Hoek, A.

AU - Müller, K. E.

PY - 1996/8

Y1 - 1996/8

N2 - The reactivity of monoclonal antibodies entered in the panels of the Third Workshop on Ruminant Leucocyte Antigens with lymphocytes and monocytes of normal and β2-integrin deficient (Bovine Leucocyte Adhesion Deficiency: BLAD) animals was determined by flow cytometry to investigate potential specificities to α- or β-chains of β2-integrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but three had reactivity with cells from BLAD animals. We conclude that our approach provides an easy way to reliably identify the majority of β2-integrin specific monoclonal antibodies.

AB - The reactivity of monoclonal antibodies entered in the panels of the Third Workshop on Ruminant Leucocyte Antigens with lymphocytes and monocytes of normal and β2-integrin deficient (Bovine Leucocyte Adhesion Deficiency: BLAD) animals was determined by flow cytometry to investigate potential specificities to α- or β-chains of β2-integrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but three had reactivity with cells from BLAD animals. We conclude that our approach provides an easy way to reliably identify the majority of β2-integrin specific monoclonal antibodies.

KW - BLAD

KW - Cattle

KW - Leucocytes

KW - Monoclonal antibodies

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U2 - 10.1016/0165-2427(96)05585-7

DO - 10.1016/0165-2427(96)05585-7

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C2 - 8896223

AN - SCOPUS:0030221198

SN - 0165-2427

VL - 52

SP - 341

EP - 345

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 4

ER -

Identification of monoclonal antibodies with specificity to α- or β-chains of β₂-integrins using peripheral blood leucocytes of normal and Bovine Leucocyte Adhesion Deficient (BLAD) cattle

Abstract

Keywords

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