Identification of cambium stem cell factors and their positioning mechanism

Gugan Eswaran; Xixi Zhang; Jacob Pieter Rutten; Jingyi Han; Hiroyuki Iida; Jennifer López Ortiz; Riikka Mäkilä; Brecht Wybouw; Benjamin Planterose Jiménez; Leo Vainio; Alexis Porcher; Marina Leal Gavarron; Jing Zhang; Tiina Blomster; Xin Wang; David Dolan; Ondřej Smetana; Siobhán M. Brady; Melis Kucukoglu Topcu; Kirsten Ten Tusscher; J. Peter Etchells; Ari Pekka Mähönen

doi:10.1126/science.adj8752

Identification of cambium stem cell factors and their positioning mechanism

Gugan Eswaran, Xixi Zhang, Jacob Pieter Rutten, Jingyi Han, Hiroyuki Iida, Jennifer López Ortiz, Riikka Mäkilä, Brecht Wybouw, Benjamin Planterose Jiménez, Leo Vainio, Alexis Porcher, Marina Leal Gavarron, Jing Zhang, Tiina Blomster, Xin Wang, David Dolan, Ondřej Smetana, Siobhán M. Brady, Melis Kucukoglu Topcu, Kirsten Ten Tusscher^*J. Peter Etchells^*, Ari Pekka Mähönen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

Original language	English
Pages (from-to)	646-653
Number of pages	8
Journal	Science (New York, N.Y.)
Volume	386
Issue number	6722
DOIs	https://doi.org/10.1126/science.adj8752
Publication status	Published - 8 Nov 2024

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Eswaran, G., Zhang, X., Rutten, J. P., Han, J., Iida, H., López Ortiz, J., Mäkilä, R., Wybouw, B., Planterose Jiménez, B., Vainio, L., Porcher, A., Leal Gavarron, M., Zhang, J., Blomster, T., Wang, X., Dolan, D., Smetana, O., Brady, S. M., Kucukoglu Topcu, M., ... Mähönen, A. P. (2024). Identification of cambium stem cell factors and their positioning mechanism. Science (New York, N.Y.), 386(6722), 646-653. https://doi.org/10.1126/science.adj8752

@article{62cbe165cd7f4e42b42775b850200b36,

title = "Identification of cambium stem cell factors and their positioning mechanism",

abstract = "Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.",

author = "Gugan Eswaran and Xixi Zhang and Rutten, {Jacob Pieter} and Jingyi Han and Hiroyuki Iida and {L{\'o}pez Ortiz}, Jennifer and Riikka M{\"a}kil{\"a} and Brecht Wybouw and {Planterose Jim{\'e}nez}, Benjamin and Leo Vainio and Alexis Porcher and {Leal Gavarron}, Marina and Jing Zhang and Tiina Blomster and Xin Wang and David Dolan and Ond{\v r}ej Smetana and Brady, {Siobh{\'a}n M.} and {Kucukoglu Topcu}, Melis and {Ten Tusscher}, Kirsten and Etchells, {J. Peter} and M{\"a}h{\"o}nen, {Ari Pekka}",

year = "2024",

month = nov,

day = "8",

doi = "10.1126/science.adj8752",

language = "English",

volume = "386",

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Eswaran, G, Zhang, X, Rutten, JP, Han, J, Iida, H, López Ortiz, J, Mäkilä, R, Wybouw, B, Planterose Jiménez, B, Vainio, L, Porcher, A, Leal Gavarron, M, Zhang, J, Blomster, T, Wang, X, Dolan, D, Smetana, O, Brady, SM, Kucukoglu Topcu, M, Ten Tusscher, K, Etchells, JP & Mähönen, AP 2024, 'Identification of cambium stem cell factors and their positioning mechanism', Science (New York, N.Y.), vol. 386, no. 6722, pp. 646-653. https://doi.org/10.1126/science.adj8752

TY - JOUR

T1 - Identification of cambium stem cell factors and their positioning mechanism

AU - Eswaran, Gugan

AU - Zhang, Xixi

AU - Rutten, Jacob Pieter

AU - Han, Jingyi

AU - Iida, Hiroyuki

AU - López Ortiz, Jennifer

AU - Mäkilä, Riikka

AU - Wybouw, Brecht

AU - Planterose Jiménez, Benjamin

AU - Vainio, Leo

AU - Porcher, Alexis

AU - Leal Gavarron, Marina

AU - Zhang, Jing

AU - Blomster, Tiina

AU - Wang, Xin

AU - Dolan, David

AU - Smetana, Ondřej

AU - Brady, Siobhán M.

AU - Kucukoglu Topcu, Melis

AU - Ten Tusscher, Kirsten

AU - Etchells, J. Peter

AU - Mähönen, Ari Pekka

PY - 2024/11/8

Y1 - 2024/11/8

N2 - Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

AB - Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

UR - http://www.scopus.com/inward/record.url?scp=85209165432&partnerID=8YFLogxK

U2 - 10.1126/science.adj8752

DO - 10.1126/science.adj8752

M3 - Article

C2 - 39509505

AN - SCOPUS:85209165432

SN - 0036-8075

VL - 386

SP - 646

EP - 653

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6722

ER -

Identification of cambium stem cell factors and their positioning mechanism

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