Identification of an l-Arabitol Transporter from Aspergillus niger

Jiali Meng; Miia R Mäkelä; Ronald P de Vries

doi:10.3390/biom13020188

Identification of an l-Arabitol Transporter from Aspergillus niger

Jiali Meng, Miia R Mäkelä, Ronald P de Vries^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.

Original language	English
Article number	188
Pages (from-to)	1-11
Number of pages	11
Journal	Biomolecules
Volume	13
Issue number	2
DOIs	https://doi.org/10.3390/biom13020188
Publication status	Published - Feb 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Funding

This research was funded by the Chinese Scholarship Council (CSC), grant number CSC201907720027 to J.M. and Novo Nordisk Foundation, grant number NNF21OC0067087 to M.R.M.

Funders	Funder number
China Scholarship Council	CSC201907720027
Novo Nordisk Fonden	NNF21OC0067087

Keywords

Aspergillus niger
l-arabitol
polyol
sugar beet pulp
transporter
wheat bran

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10.3390/biom13020188Licence: CC BY

biomolecules-13-00188Final published version, 2.19 MBLicence: CC BY

Cite this

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title = "Identification of an l-Arabitol Transporter from Aspergillus niger",

abstract = "l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.",

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doi = "10.3390/biom13020188",

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AU - Meng, Jiali

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

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N2 - l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.

AB - l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.

KW - Aspergillus niger

KW - l-arabitol

KW - polyol

KW - sugar beet pulp

KW - transporter

KW - wheat bran

UR - https://www.scopus.com/pages/publications/85149018365

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DO - 10.3390/biom13020188

M3 - Article

C2 - 36830558

SN - 2218-273X

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ER -

Identification of an l-Arabitol Transporter from Aspergillus niger

Abstract

Bibliographical note

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Keywords

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