Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant

Elsa Berends, Ludwig Lehle, Maurice Henquet, Thamara Hesselink, Han A.B. Wösten, Luis G. Lugones, Dirk Bosch

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man5GlcNAc2-PP-Dol resulting in the formation of Man6GlcNAc2-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc3Man9GlcNAc 2 for N-glycosylation of proteins. Here, we identified the alg3 gene of the mushroom-forming fungus Schizophyllum commune by homology search. Its function was confirmed by the complementation of the Δalg3 strain of S. cerevisiae. Inactivation of alg3 in S. commune resulted in the production of predominantly Man3GlcNAc2 protein-linked N-glycans. No impact on growth nor a developmental phenotype due to the deletion was observed. This provides a first step toward engineering of a homogeneous, humanized N-glycosylation pattern for the production of therapeutic glycoproteins in mushrooms. © 2012 The Author.
    Original languageEnglish
    Pages (from-to)147-154
    Number of pages8
    JournalGlycobiology
    Volume23
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2013

    Keywords

    • alg3
    • basidiomycete
    • fungus
    • glycosylation
    • Schizophyllum commune
    • Alg3 protein
    • fungal protein
    • oligosaccharide
    • unclassified drug
    • article
    • fungal gene
    • fungal strain
    • mushroom
    • nonhuman
    • phenotype
    • priority journal
    • protein glycosylation

    Fingerprint

    Dive into the research topics of 'Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant'. Together they form a unique fingerprint.

    Cite this