Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant

Elsa Berends, Ludwig Lehle, Maurice Henquet, Thamara Hesselink, Han A.B. Wösten, Luis G. Lugones, Dirk Bosch

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man5GlcNAc2-PP-Dol resulting in the formation of Man6GlcNAc2-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc3Man9GlcNAc 2 for N-glycosylation of proteins. Here, we identified the alg3 gene of the mushroom-forming fungus Schizophyllum commune by homology search. Its function was confirmed by the complementation of the Δalg3 strain of S. cerevisiae. Inactivation of alg3 in S. commune resulted in the production of predominantly Man3GlcNAc2 protein-linked N-glycans. No impact on growth nor a developmental phenotype due to the deletion was observed. This provides a first step toward engineering of a homogeneous, humanized N-glycosylation pattern for the production of therapeutic glycoproteins in mushrooms. © 2012 The Author.
Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalGlycobiology
Volume23
Issue number2
DOIs
Publication statusPublished - 1 Feb 2013

Keywords

  • alg3
  • basidiomycete
  • fungus
  • glycosylation
  • Schizophyllum commune
  • Alg3 protein
  • fungal protein
  • oligosaccharide
  • unclassified drug
  • article
  • fungal gene
  • fungal strain
  • mushroom
  • nonhuman
  • phenotype
  • priority journal
  • protein glycosylation

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